NewRingeisen Erin, Jordahl Jacy, Bowers Lisa
St. Olaf College, Northfield, Minnesota, United States.
MicroPubl Biol. 2025 Feb 28;2025. doi: 10.17912/micropub.biology.001457. eCollection 2025.
Gram-negative bacteria have outer membrane proteins called TonB-dependent receptors (TBDRs) that facilitate energy-dependent transport of substrates. is a gram-negative bacterium with a large set of TBDRs, yet the function of many of these TBDRs remains uncharacterized. This study focuses on SucA, a TBDR that transports sucrose. Previous studies showed that expression was induced in the presence of sucrose, yet did not provide a measurable fitness advantage under the conditions tested. This work identifies conditions where does confer a significant growth advantage and provides evidence that SucA activity relies on the proton motive force, a feature of canonical TBDRs.
革兰氏阴性菌具有称为托品B依赖性受体(TBDRs)的外膜蛋白,这些蛋白有助于底物的能量依赖性转运。[具体细菌名称]是一种具有大量TBDRs的革兰氏阴性菌,但其中许多TBDRs的功能仍未明确。本研究聚焦于转运蔗糖的TBDR SucA。先前的研究表明,[具体细菌名称]在蔗糖存在下会被诱导表达,但在测试条件下并未提供可测量的适应性优势。这项工作确定了[具体细菌名称]确实赋予显著生长优势的条件,并提供证据表明SucA的活性依赖于质子动力势,这是典型TBDRs的一个特征。