采用气相色谱 - 质谱联用技术对脑脊液和脑组织中的γ-氨基丁酸进行特异性和灵敏性测定。
A specific and sensitive determination of gamma aminobutyric acid in CSF and brain tissue by gas chromatography-mass spectrometry.
作者信息
Schaaf J M, Teelken A W, Muskiet F A, Nagel G, Wolthers B G
出版信息
J Neurosci Methods. 1985 May;13(3-4):257-65. doi: 10.1016/0165-0270(85)90074-3.
The determination of gamma-aminobutyric acid (GABA) in cerebrospinal fluid and brain extracts is described. Its heptafluorobutyryl-isobutanol derivative was measured both by electron impact and chemical ionization mass fragmentography using GABA-d6 as internal standard. The derivatization product is stable for several days. The method is sensitive (1 ng absolute in cerebrospinal fluid and 30 pg in standard GABA solutions) and specific, when chemical ionization mode is applied. Normal values of GABA are in rat brain extracts (1.40 +/- 0.32 mumol/g fresh weight) and human CSF (18.3 +/- 10.0 ng/ml).
本文描述了脑脊液和脑提取物中γ-氨基丁酸（GABA）的测定方法。以GABA-d6作为内标，通过电子轰击和化学电离质谱碎片分析法测定其七氟丁酰-异丁醇衍生物。衍生化产物在数天内稳定。当采用化学电离模式时，该方法灵敏（脑脊液中绝对检测限为1 ng，标准GABA溶液中为30 pg）且具有特异性。大鼠脑提取物中GABA的正常值为（1.40±0.32 μmol/g鲜重），人脑脊液中为（18.3±10.0 ng/ml）。
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