犬血浆中碳酸酐酶的抑制作用。

Inhibition of carbonic anhydrase in dog plasma.

作者信息

Rispens P, Hessels J, Zwart A, Zijlstra W G

出版信息

Pflugers Arch. 1985 Apr;403(4):344-7. doi: 10.1007/BF00589244.

Abstract

Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. C1- inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.

摘要

采用pH稳态法，我们在存在不同量犬血浆的情况下测量了犬红细胞碳酸酐酶（CA）的活性。一种血浆因子似乎能够抑制约86%的总CA活性，这与CA II的相对活性相对应。碘乙酸钠被证明可抑制总CA活性高达约13%，这与CA I的相对活性相对应。Cl-可抑制总CA活性高达约20%，推测主要是通过其对CA I的强烈影响。得出的结论是，溶血程度高达3%时，不会出现明显的血浆CA活性。