Wang Yanan, Qian Xinyi, Chen Lingzhi, Yong Yidan, Wu Mengmeng, Li Yihao, Ni Zaizhong, Li Lulu, Shao Ying, Chen Anhui
College of Food and Bioengineering, Xuzhou University of Technology, 221018 Xuzhou, Jiangsu, China; Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, 221018 Xuzhou, Jiangsu, China.
College of Food and Bioengineering, Xuzhou University of Technology, 221018 Xuzhou, Jiangsu, China.
Int J Biol Macromol. 2025 May;307(Pt 4):142294. doi: 10.1016/j.ijbiomac.2025.142294. Epub 2025 Mar 19.
As natural bioactive compounds, polysaccharides have promising effects in the treatment of type 2 diabetes mellitus (T2DM) owing to their changes to the intestinal microenvironment; however, the mechanisms underlying their effects have not been elucidated. In the present study, a polysaccharide LV-P4-1 isolated from Lactaruis volemus Fr was purified and characterized. The hyperglycemic function and the regulatory effect of LV-P4-1 on the gut microbiota and its metabolites were investigated in a T2DM mouse model. LV-P4-1 was mainly consisted of Fuc, Gal, Glc, Man, and GlcA, with a molecular weight of 5.89 kDa. The functional groups and glycosyl linkage types of LV-P4-1 were investigated using Fourier transform infrared spectroscopy, methylation and nuclear magnetic resonance analyses. Oral administration of 400 mg/kg LV-P4-1 increased glucose metabolism and alleviated tissue damage in mice with T2DM. Moreover, LV-P4-1 significantly regulated the abundances of gut microbiota, changed metabolite levels, and altered some metabolic pathways involved in T2DM development. Spearman analysis showed that the alterations in the gut microbiota were closely related to the differential metabolites. These results suggest that LV-P4-1 may alleviate hyperglycemia by influencing the structure of the intestinal microbiota and regulating the metabolic profile by altering the activity of certain metabolic pathways.
作为天然生物活性化合物，多糖因其对肠道微环境的改变而在2型糖尿病（T2DM）治疗中具有潜在作用；然而，其作用机制尚未阐明。在本研究中，对从松乳菇中分离得到的多糖LV-P4-1进行了纯化和表征。在T2DM小鼠模型中研究了LV-P4-1的降血糖功能及其对肠道微生物群及其代谢产物的调节作用。LV-P4-1主要由岩藻糖、半乳糖、葡萄糖、甘露糖和葡萄糖醛酸组成，分子量为5.89 kDa。采用傅里叶变换红外光谱、甲基化和核磁共振分析等方法研究了LV-P4-1的官能团和糖基连接类型。口服400 mg/kg LV-P4-1可增加T2DM小鼠的葡萄糖代谢并减轻组织损伤。此外，LV-P4-1显著调节肠道微生物群的丰度，改变代谢产物水平，并改变一些与T2DM发生发展相关的代谢途径。Spearman分析表明，肠道微生物群的改变与差异代谢产物密切相关。这些结果表明，LV-P4-1可能通过影响肠道微生物群结构和改变某些代谢途径的活性来调节代谢谱，从而减轻高血糖。
