Qu Xiaolong, Meng Zongwu, Zhang Tao, Dai Heng, Wu Pian, Ding Ping
Xiangya School of Public Health, Central South University, Changsha, 410078, Hunan, China.
Hunan Provincial Key Laboratory of Clinical Epidemiology, Changsha, Hunan, 410078, PR China.
Mikrochim Acta. 2025 Mar 25;192(4):255. doi: 10.1007/s00604-025-07111-2.
The simple, rapid, and simultaneous detection of multiple foodborne pathogens in food is crucial for ensuring public safety. In this study, a rational design strategy for lanthanide-based metal-organic frameworks (Ln-MOFs), informed by theoretical calculations, was proposed. The calculated results were experimentally verified to screen out the optimal Ln-MOF for fluorescence efficiency. The selected Ln-MOFs were coupled with phages that exhibit specific pathogen recognition to develop phage@Ln-MOF fluorescent probes, while the magnetic nanoparticles were conjugated with phages to form capture probes. On this basis, a fluorescent biosensor was developed for the simultaneous detection of three major foodborne pathogens-Escherichia coli (E. coli), Staphylococcus aureus (S. aureus), and Salmonella. This sensor facilitated the detection of all three pathogens within 15 min, with limit of detection (LOD) as low as 1 CFU/mL. Moreover, this fluorescent biosensor was compatible with on-site visual detection, utilizing a self-designed portable dark box and smartphone-assisted visualization, achieving an LOD of approximately 1-2 CFU/mL for E. coli, S. aureus, and Salmonella. This work demonstrates a novel approach for the rapid on-site detection of multiple foodborne pathogens, which holds promise for advancing field-ready diagnostic tools in food safety monitoring.
食品中多种食源性病原体的简单、快速及同步检测对于确保公共安全至关重要。在本研究中,提出了一种基于理论计算的镧系金属有机框架(Ln-MOFs)的合理设计策略。计算结果经实验验证,以筛选出荧光效率最佳的Ln-MOF。将所选的Ln-MOF与表现出特定病原体识别能力的噬菌体偶联,开发出噬菌体@Ln-MOF荧光探针,同时将磁性纳米颗粒与噬菌体偶联形成捕获探针。在此基础上,开发了一种荧光生物传感器,用于同时检测三种主要食源性病原体——大肠杆菌(E. coli)、金黄色葡萄球菌(S. aureus)和沙门氏菌。该传感器在15分钟内即可完成对所有三种病原体的检测,检测限(LOD)低至1 CFU/mL。此外,这种荧光生物传感器与现场视觉检测兼容,利用自行设计的便携式暗箱和智能手机辅助可视化,对大肠杆菌、金黄色葡萄球菌和沙门氏菌的LOD约为1-2 CFU/mL。这项工作展示了一种快速现场检测多种食源性病原体的新方法,有望推动食品安全监测中现场可用诊断工具的发展。