Okane Izumi, Kurita Akiko, Ono Yoshitaka
Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572, Japan.
Agro-Bioresources Science and Technology, University of Tsukuba, Tsukuba 305-8572, Japan.
J Fungi (Basel). 2025 Mar 3;11(3):193. doi: 10.3390/jof11030193.
Grapevine leaf rust (GLR) in temperate Asia is caused by and ; the former is commonly found on commercial grape cultivars ( spp.) and the latter on a wild grape species, . The two GLR fungi were found to co-occur in at two survey sites in Japan. Under experimental conditions, both fungi parasitize and develop into uredinial and telial stages on commercial grape cultivars and wild species. Despite the assumed involvement of in GLR symptoms in commercial vineyards, there has been no confirmed report of its incidence, and it is not clear whether commonly occurs on under natural conditions. In this study, we aimed to disclose the occurrence and, in particular, the co-occurrence of the two species in a wide array of commercial grape cultivars and in Japan based on the detection of targeted DNA markers with specific PCR primer pairs. This study confirmed the occurrence of only infection in symptomatic samples of grape cultivars, while the co-occurrence was observed only in . was widely detected in specimens.
温带亚洲的葡萄叶锈病（GLR）由 和 引起；前者常见于商业葡萄品种（ 种），后者见于一种野生葡萄品种， 。在日本的两个调查地点发现这两种葡萄叶锈病真菌同时存在。在实验条件下，这两种真菌都能寄生并在商业葡萄品种和野生品种上发育成夏孢子阶段和冬孢子阶段。尽管推测 在商业葡萄园的葡萄叶锈病症状中起作用，但尚未有其发病率的确切报告，也不清楚 在自然条件下是否常见于 。在本研究中，我们旨在通过使用特异性PCR引物对检测靶向DNA标记，揭示这两种物种在日本多种商业葡萄品种和 中的发生情况，特别是它们的同时存在情况。本研究证实，在葡萄品种的有症状样本中仅检测到 感染，而仅在 中观察到两者同时存在。在 标本中广泛检测到 。
