Reindorf C A, Kurantsin-Mills J, Allotey J B, Castro O
Am J Hematol. 1985 Jul;19(3):229-36. doi: 10.1002/ajh.2830190304.
The effects of oxygenated perfluorotributylamine (Fluosol-43) on the rheological properties of sickle (HbSS) erythrocytes have been determined by means of microviscometry and positive pressure cell filtration. Incubation of deoxygenated sickled erythrocytes (pO2 congruent to 30 mmHg) with oxygenated Fluosol-43 reduced the percentage of sickled erythrocytes from about 63 to 33%. Deoxygenation of 40% suspension of sickle erythrocytes in autologous plasma increased the viscosity by about 160% at shear rate of 1.15 sec-1. Incubation of the deoxygenated sickled erythrocytes with oxygenated Fluosol-43 significantly reduced the viscosity at the low shear rates. Filtration of 0.2% suspension of deoxygenated sickle erythrocytes through capillary-sized Nuclepore filters showed high resistance at low flow rates. Oxygenated Fluosol-43 increased the deformability of HbSS erythrocytes and thereby reduced the resistance at flow rates less than 1 ml/min. These data suggest that perfluorocarbons may be useful in reducing the propensity of hemoglobin S polymerization and sickling and thereby prevent tissue infarction in vaso-occlusive crisis. Therefore, the concept of examining the potential application of perfluorochemicals for alleviating severe vaso-occlusive events may be useful.
通过微粘度测定法和正压细胞过滤法，已确定了含氧全氟三丁胺（氟碳乳剂-43）对镰状（HbSS）红细胞流变学特性的影响。将脱氧镰状红细胞（pO2约为30 mmHg）与含氧氟碳乳剂-43一起孵育，可使镰状红细胞的百分比从约63%降至33%。镰状红细胞在自体血浆中的40%悬浮液脱氧后，在剪切速率为1.15秒-1时，粘度增加了约160%。将脱氧镰状红细胞与含氧氟碳乳剂-43一起孵育，在低剪切速率下可显著降低粘度。通过毛细管大小的核孔滤器对0.2%脱氧镰状红细胞悬浮液进行过滤，在低流速下显示出高阻力。含氧氟碳乳剂-43增加了HbSS红细胞的变形能力，从而降低了流速低于1毫升/分钟时的阻力。这些数据表明，全氟化碳可能有助于降低血红蛋白S聚合和镰状化的倾向，从而预防血管闭塞性危机中的组织梗死。因此，研究全氟化合物在缓解严重血管闭塞事件方面潜在应用的概念可能是有用的。
