脑脑苷脂：使用新型色谱支持物——镁硅土进行制备性纯化
Brain cerebrosides: preparative purification using a new chromatographic support--magnammsil.
作者信息
Kant K, Hallen R M
出版信息
Anal Biochem. 1985 Jun;147(2):455-7. doi: 10.1016/0003-2697(85)90297-0.
PMID:4014690
Abstract
A preparative method for isolation and purification of cerebrosides from bovine brain is described. A crude cerebroside fraction is precipitated with ether from a chloroform-methanol extract and purification is achieved by column chromatography on a new column support, "magnammsil." A partial separation of hydroxy and nonhydroxy cerebrosides is achieved. The method is more economical for a large-scale preparation than previously published methods.
摘要
本文描述了一种从牛脑中分离和纯化脑苷脂的制备方法。粗脑苷脂级分从氯仿 - 甲醇提取物中用乙醚沉淀，通过在新型柱填料“magnammsil”上进行柱色谱法实现纯化。羟基脑苷脂和非羟基脑苷脂得到了部分分离。该方法对于大规模制备而言比先前发表的方法更经济。
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