Kochetova E S, Matveeva D K, Melik-Pashayev A E, Andreeva E R, Romanov Yu A, Buravkova L B
Institute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
Lomonosov Moscow State University, Moscow, Russia.
Bull Exp Biol Med. 2025 Feb;178(4):535-540. doi: 10.1007/s10517-025-06369-z. Epub 2025 Mar 29.
To obtain extracellular matrix (ECM) of umbilical cord multipotent mesenchymal stromal cells (UC-MSCs), six decellularization protocols were used, based on physical (freeze/thaw) and chemical (Triton-X100, CHAPS, SDS, and NP-40) methods. The quality of decellularization was assessed using phase-contrast and fluorescence microscopy, quantitative image analysis was performed as well. It was shown that protocols based on CHAPS/NH4OH and Triton X-100/NH4OH are optimal for decellularizing UC-MSC layers and provide high-quality ECM. The SDS-based protocol may be useful when certain requirements to matrix structure are specified. The presented data will be in demand for the development of biomedical products.
为了获得脐带多能间充质基质细胞（UC-MSCs）的细胞外基质（ECM），基于物理方法（冻融）和化学方法（Triton-X100、CHAPS、SDS和NP-40）使用了六种去细胞方案。使用相差显微镜和荧光显微镜评估去细胞的质量，并进行定量图像分析。结果表明，基于CHAPS/NH4OH和Triton X-100/NH4OH的方案对于UC-MSC层的去细胞是最佳的，并能提供高质量的ECM。当对基质结构有特定要求时，基于SDS的方案可能有用。所呈现的数据将在生物医学产品的开发中具有需求。
