3号和7号染色体的染色体畸变以及P16突变与涎腺肿瘤恶性程度的相关性
Association of chromosomal aberrations in chromosomes 3 and 7, and P16 mutations with malignancy in salivary gland tumors.
作者信息
Wu Yanxia, Xie Zhiyue, Peng Nan, Zhou Rui, Zhao Liang
机构信息
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China.
Department of Pathology, Guangdong Province Key Laboratory of Molecular Tumor Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, Guangdong, China.
出版信息
PeerJ. 2025 Mar 31;13:e19217. doi: 10.7717/peerj.19217. eCollection 2025.
BACKGROUND
Salivary gland tumors, a prevalent type of head and neck neoplasm, exhibit significant morphological diversity and overlapping features, complicating pathological diagnosis. Although fluorescence hybridization (FISH) is widely used for tumor detection, its diagnostic utility in salivary gland tumors remains unclear. This study aimed to explore a novel FISH-based approach to differentiate benign from malignant salivary gland tumors.
METHODS
Gene probes (CSP3, CSP7, and GSP P16) were designed to detect P16 gene deletion, and polysomies of chromosomes 3 and 7. The FISH analysis was conducted on 22 malignant and 12 benign salivary gland tumor samples with complete clinical data. The study was expanded to 78 samples for further validation.
RESULTS
The CSP3, CSP7, and GSP P16 probes exhibited high specificity for salivary gland tumors, though CSP7 exhibited lower sensitivity. The combination of CSP3 and GSP P16 probes outperformed single-probe analysis or other probe combinations.
CONCLUSION
The CSP3 and GSP P16 probe combination provides a highly sensitive and specific method for distinguishing malignant from benign salivary gland tumors.
背景
唾液腺肿瘤是头颈部肿瘤中常见的一种，具有显著的形态学多样性和重叠特征，使病理诊断复杂化。尽管荧光原位杂交（FISH）广泛用于肿瘤检测，但其在唾液腺肿瘤中的诊断效用仍不明确。本研究旨在探索一种基于FISH的新方法来区分良性和恶性唾液腺肿瘤。
方法
设计基因探针（CSP3、CSP7和GSP P16）以检测P16基因缺失以及3号和7号染色体的多体性。对22例具有完整临床数据的恶性唾液腺肿瘤样本和12例良性唾液腺肿瘤样本进行FISH分析。该研究扩展至78个样本以进行进一步验证。
结果
CSP3、CSP7和GSP P16探针对唾液腺肿瘤具有高特异性，尽管CSP7的敏感性较低。CSP3和GSP P16探针的组合优于单探针分析或其他探针组合。
结论
CSP3和GSP P16探针组合为区分良性和恶性唾液腺肿瘤提供了一种高度敏感和特异的方法。
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