Yang Jiajia, Wang Xuan, Chen Ying, He Ye, Li Ping, Wen Xin, Wang Bi
College of Animal Science, Guizhou University, Guiyang, Guizhou Province 550025, PR China; Key Laboratory of Animal Diseases and Veterinary Public Health of Guizhou Province (Cultivation), Guiyang, Guizhou Province 550025, PR China.
Institute of Animal Husbandry and Veterinary Medicine of Guizhou Academy of Agricultural Sciences, Guizhou Province 550025, PR China.
Vet J. 2025 Jun;311:106343. doi: 10.1016/j.tvjl.2025.106343. Epub 2025 Apr 3.
Cecal coccidiosis is a severe and lethal parasitic disease affecting chickens, making the search for effective preventive agents free of contamination and drug resistance crucial for controlling this condition in poultry. Previous studies have demonstrated that the ChangQing compound has significant therapeutic effects against cecal coccidiosis; however, its potential as a preventive measure has yet to be evaluated. In this study, we established an experimental model for the prevention of cecal coccidiosis in chickens using the ChangQing compound for the first time. A comprehensive evaluation was performed on survival rates, relative weight gain, oocyst production, the anticoccidial index (ACI), immune parameters, parasitic tissue pathology, and microbial diversity in cecal contents. Results indicated that the ChangQing compound at a concentration of 5.0 g/L achieved an ACI of 178.10 in the Pre-Exposure Prophylaxis (PrEP) group and 173.12 in the Post-Exposure Prophylaxis (PEP) group. Compared to the positive control group, cecal lesions were reduced, and indices for the spleen, liver, and bursa of Fabricius increased in both experimental groups following ChangQing administration. Furthermore, levels of immune factors, IgA, IgG, and IgM significantly elevated. The abundance of beneficial bacteria, including Lactobacillus, Bacteroides, and Alistipes, increased in the 5.0 g/L ChangQing compound group, while potential pathogens like Escherichia-Shigella, Enterococcus, and norank_f_Oscillospiraceae were reduced. These findings offer critical data for coccidiosis prevention in chickens and lay a theoretical foundation for future research on the antiparasitic mechanisms of traditional Chinese medicine.
盲肠球虫病是一种严重的致死性寄生虫病，影响着鸡群，因此寻找无污染且无耐药性的有效预防药物对于控制家禽的这种疾病至关重要。先前的研究表明，长青复方对盲肠球虫病具有显著的治疗效果；然而，其作为预防措施的潜力尚未得到评估。在本研究中，我们首次使用长青复方建立了鸡盲肠球虫病预防的实验模型。对存活率、相对体重增加、卵囊产量、抗球虫指数（ACI）、免疫参数、寄生组织病理学以及盲肠内容物中的微生物多样性进行了全面评估。结果表明，浓度为5.0 g/L的长青复方在暴露前预防（PrEP）组中的ACI为178.10，在暴露后预防（PEP）组中的ACI为173.12。与阳性对照组相比，给药长青复方后，两个实验组的盲肠病变均减少，脾脏、肝脏和法氏囊的指数均增加。此外，免疫因子IgA、IgG和IgM的水平显著升高。在5.0 g/L长青复方组中，包括乳酸杆菌、拟杆菌和艾利斯菌属在内的有益菌丰度增加，而大肠杆菌-志贺氏菌属、肠球菌和无分类阶元的颤螺菌科等潜在病原体减少。这些发现为鸡球虫病的预防提供了关键数据，并为未来中药抗寄生虫机制的研究奠定了理论基础。
