Aljuhani Wafaa, Wylie Matthew P, Purusottam Rudra N, McCoy Colin P, Bell Steven E J
School of Chemistry and Chemical Engineering, Queen's University Belfast, Belfast BT9 5AG, U.K.
School of Pharmacy, Queen's University Belfast, Belfast BT9 7BL, U.K.
Biomacromolecules. 2025 May 12;26(5):2900-2908. doi: 10.1021/acs.biomac.4c01707. Epub 2025 Apr 6.
Understanding the interplay between nanoparticles, biomaterials and drug molecules in biological environments is important but studying these interactions in complex systems such as biofilms is challenging. Here, surface-enhanced Raman spectroscopy (SERS) with gold nanostars (NS) was used to monitor how biofilm components influence the binding and SERS signals of two antibiotics, levofloxacin (Levo) and ampicillin (Amp). The SERS signals of both antibiotics were reduced by approximately 70% (Levo) and 90% (Amp) in biofilm environments. Investigations of mixtures of model biofilm components: adenine (nucleic acids), alginate (polysaccharides) and albumin (proteins), revealed that their interactions with NS are governed by coupled equilibria. This gave surprising results, for example, alginate reduced the interference from adenine and albumin, so adding alginate increased the intensity of the antibiotic signals 4x. These findings highlight the importance of matrix component interactions in modulating detection sensitivity and show that these effects are critical in allowing clinically relevant detection levels to be achieved.
了解纳米颗粒、生物材料和药物分子在生物环境中的相互作用非常重要，但在生物膜等复杂系统中研究这些相互作用具有挑战性。在这里，使用带有金纳米星（NS）的表面增强拉曼光谱（SERS）来监测生物膜成分如何影响两种抗生素左氧氟沙星（Levo）和氨苄青霉素（Amp）的结合及SERS信号。在生物膜环境中，两种抗生素的SERS信号分别降低了约70%（Levo）和90%（Amp）。对模型生物膜成分混合物（腺嘌呤（核酸）、藻酸盐（多糖）和白蛋白（蛋白质））的研究表明，它们与NS的相互作用受耦合平衡支配。这产生了令人惊讶的结果，例如，藻酸盐减少了腺嘌呤和白蛋白的干扰，因此添加藻酸盐使抗生素信号强度增加了4倍。这些发现突出了基质成分相互作用在调节检测灵敏度方面的重要性，并表明这些效应对于实现临床相关检测水平至关重要。
