Matsuzawa Tomohiko, Shimada Naoki, Fujiwa Shimma
1 Department of Applied Biological Science, Faculty of Agriculture, Kagawa University.
J Appl Glycosci (1999). 2025 Feb 20;72(1):7201202. doi: 10.5458/jag.7201202. eCollection 2025.
Isoprimeverose [α-D-xylopyranosyl-(1→6)-D-glucose] is produced from xyloglucan using the cooperative action of glycoside hydrolases including isoprimeverose-producing oligoxyloglucan hydrolase and β-galactosidase in . This study investigated strains and culture conditions suitable for isoprimeverose production from xyloglucan. Each strain of had a different ability to degrade xyloglucans. When an strain with high xyloglucan-degradation activity was cultured in a medium containing partially degraded xyloglucan as the carbon source, the production of glycoside hydrolases that degrade xyloglucan into isoprimeverose was highly induced. Our procedure efficiently produced isoprimeverose from xyloglucan without any genetically modified microorganisms or purification of enzymes.
异樱草糖[α-D-吡喃木糖基-(1→6)-D-葡萄糖]是利用糖苷水解酶（包括产异樱草糖的低聚木葡聚糖水解酶和β-半乳糖苷酶）的协同作用从木葡聚糖中产生的。本研究调查了适合从木葡聚糖生产异樱草糖的菌株和培养条件。每种菌株降解木葡聚糖的能力不同。当具有高木葡聚糖降解活性的菌株在含有部分降解的木葡聚糖作为碳源的培养基中培养时，将木葡聚糖降解为异樱草糖的糖苷水解酶的产生被高度诱导。我们的方法无需任何基因改造微生物或酶的纯化就能从木葡聚糖高效生产异樱草糖。
