Mutlu Ahmet, Bakici Burcu, Gunduz Ayşe Yasemin, Erinc Murat, Bulut Erdogan, Ersoy Onur, Bayraktar Hakan, Kalcioglu Mahmut Tayyar
Department of Otorhinolaryngology, Istanbul Medeniyet University Faculty of Medicine, İstanbul, Türkiye.
Department of Otorhinolaryngology, Göztepe Prof. Dr. Süleyman Yalçın City Hospital, İstanbul, Türkiye.
J Int Adv Otol. 2025 Mar 25;21(2):1-9. doi: 10.5152/iao.2025.241526.
Endoscopes play an important role in otologic surgery. Baby shampoos, known for their hypoallergenic, anti-irritant, and antifogging properties, are increasingly being used. The purpose of this study was to evaluate the safety and ototoxic effects of baby shampoo in an animal model using both electrophysiological and histological methods.
Twenty-eight male Sprague-Dawley rats were divided into 4 groups: 7 control (group 1), 7 saline (group 2), 7 isopropyl alcohol (70%) + chlorhexidine (2%) antiseptic (group 3), and 7 baby shampoo (group 4). Baseline hearing was assessed by auditory brainstem response (ABR). All groups except the control received 3 doses of their respective substances by intratympanic injection. Auditory brainstem response measurements were performed on day 7 and day 21, after which the temporal bones were dissected. Histological evaluation of the cochlea and vascular endothelial growth factor (VEGF) immunoreactivity studies were performed.
Baseline hearing thresholds were similar and within normal limits. On days 7 and 21, hearing thresholds were significantly impaired at all frequencies in the experimental groups. In particular, groups 3 and 4 had higher thresholds than the other groups. Baby shampoo caused significant damage to outer hair cells and sustentacular cells and decreased VEGF immunoreactivity in the stria vascularis, spiral ligament, and organ of Corti.
Baby shampoo was found to progressively impair hearing over time, indicating its potential to cause long-term ototoxicity.
内窥镜在耳科手术中发挥着重要作用。婴儿洗发水因其低过敏性、抗刺激性和防雾特性而越来越多地被使用。本研究的目的是使用电生理和组织学方法在动物模型中评估婴儿洗发水的安全性和耳毒性作用。
28只雄性Sprague-Dawley大鼠分为4组：7只作为对照组（第1组），7只注射生理盐水（第2组），7只注射70%异丙醇+2%氯己定防腐剂（第3组），7只注射婴儿洗发水（第4组）。通过听性脑干反应（ABR）评估基线听力。除对照组外，所有组均通过鼓室内注射接受3剂各自的物质。在第7天和第21天进行听性脑干反应测量，之后解剖颞骨。进行耳蜗的组织学评估和血管内皮生长因子（VEGF）免疫反应性研究。
基线听力阈值相似且在正常范围内。在第7天和第21天，实验组所有频率的听力阈值均显著受损。特别是，第3组和第4组的阈值高于其他组。婴儿洗发水对外毛细胞和支柱细胞造成了显著损伤，并降低了血管纹、螺旋韧带和柯蒂氏器中的VEGF免疫反应性。
发现婴儿洗发水会随着时间的推移逐渐损害听力，表明其具有导致长期耳毒性的潜力。
