来自土壤变形虫的溶菌酶I的纯化及特异性

Purification and specificity of bacteriolytic enzyme I from Hartmannella glebae.

作者信息

Upadhyay J M, Mares B A, Hemelt D M, Rivet P G

出版信息

Appl Environ Microbiol. 1977 Jan;33(1):1-5. doi: 10.1128/aem.33.1.1-5.1977.

DOI:10.1128/aem.33.1.1-5.1977

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC170564/

Abstract

A soil amoeba (Hartmannella glebae), when grown in conjunction with Enterobacter aerogenes and Alcaligenes faecalis, produced two enzymes. Enzyme I was purified by gel filtration on Sephadex G-100 and chromatography on diethylaminoethyl-cellulose. It is a basic protein. The analysis of the enzymic digest of the cell walls of Micrococcus lysodeikticus after reduction and acid hydrolysis showed that the enzyme cleaved the glycosidic bond between acetylmuramic acid and acetylglucosamine of the peptidoglycan moiety of the cell walls. The enzyme is identified as endo-beta-N-acetylmuramidase.

摘要

一种土壤变形虫（土壤哈特曼氏阿米巴）与产气肠杆菌和粪产碱菌共同培养时会产生两种酶。酶I通过在葡聚糖G - 100上进行凝胶过滤和在二乙氨基乙基纤维素上进行色谱法进行纯化。它是一种碱性蛋白质。对溶壁微球菌细胞壁经还原和酸水解后的酶解产物分析表明，该酶能切割细胞壁肽聚糖部分中乙酰胞壁酸和乙酰葡糖胺之间的糖苷键。该酶被鉴定为内切-β-N-乙酰胞壁酰胺酶。

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引用本文的文献

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Bacteriolytic activities of the free-living soil amoebae, Acanthamoeba castellanii, Acanthamoeba polyphaga and Hartmannella vermiformis.

Antonie Van Leeuwenhoek. 1995 Oct;68(3):237-43. doi: 10.1007/BF00871821.

2

Specificity of bacteriolytic enzyme II from a soil amoeba, Hartmannella glebae.

Appl Environ Microbiol. 1979 Sep;38(3):373-8. doi: 10.1128/aem.38.3.373-378.1979.

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Growth and bacteriolytic activity of a soil amoeba, Hartmannella glebae.

J Bacteriol. 1968 Mar;95(3):771-4. doi: 10.1128/jb.95.3.771-774.1968.

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Bacteriolytic enzyme produced by Acanthamoeba castellanii.

Acta Microbiol Pol A. 1969;1(3):155-68.

9

Purification and properties of a bacteriolytic enzyme from a soil amoeba Hartmannella glebae.

Arch Biochem Biophys. 1969 Dec;135(1):244-52. doi: 10.1016/0003-9861(69)90536-0.

10

Isolation, purification, and properties of a second bacteriolytic enzyme from Hartmannella glebae.

Arch Biochem Biophys. 1972 Apr;149(2):470-5. doi: 10.1016/0003-9861(72)90346-3.

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