Kim Jung Wook, Choi Eun Chae, Lee Kwang Jun
Division of Zoonotic and Vector Borne Diseases Research, Center for Infectious Disease Research, National Institute of Health, Korea Disease Control and Prevention Agency, Cheongju, Republic of Korea.
Osong Public Health Res Perspect. 2025 Jun;16(3):300-307. doi: 10.24171/j.phrp.2024.0319. Epub 2025 Apr 11.
This study presents the standardized protocols developed by the Clinical-Based Human Microbiome Research and Development Project (cHMP) in the Republic of Korea.
It addresses clinical metadata collection, specimen handling, DNA extraction, sequencing methods, and quality control measures for microbiome research.
The cHMP involves collecting samples from healthy individuals and patients across various body sites, including the gastrointestinal tract, oral cavity, respiratory system, urogenital tract, and skin. These standardized procedures ensure consistent data quality through controlled specimen collection, storage, transportation, DNA extraction, and sequencing. Sequencing encompasses both amplicon and whole metagenome methods, followed by stringent quality checks. The protocols conform to international guidelines, ensuring that the data generated are both reliable and comparable across microbiome studies.
The cHMP underscores the importance of methodological standardization in enhancing data integrity, reproducibility, and advancing microbiome-based research with potential applications for improving human health outcomes.
本研究展示了大韩民国基于临床的人类微生物组研究与开发项目（cHMP）制定的标准化方案。
它涉及微生物组研究的临床元数据收集、样本处理、DNA提取、测序方法和质量控制措施。
cHMP包括从健康个体和患者的各个身体部位收集样本，这些部位包括胃肠道、口腔、呼吸系统、泌尿生殖道和皮肤。这些标准化程序通过受控的样本采集、储存、运输、DNA提取和测序确保一致的数据质量。测序包括扩增子和全宏基因组方法，随后进行严格的质量检查。这些方案符合国际准则，确保所产生的数据在微生物组研究中既可靠又具有可比性。
cHMP强调了方法标准化在提高数据完整性、可重复性以及推进基于微生物组的研究方面的重要性，这些研究在改善人类健康结果方面具有潜在应用价值。
