Ozbay Ahmet Duhan, Somuncu Ahmet Mehmet, Cicek Ibrahim, Yavuzer Bulent, Bulut Seval, Huseynova Gulbaniz, Tastan Tugba Bal, Gulaboglu Mine, Suleyman Halis
Department of Ophthalmology, Erzurum Regional Training and Research Hospital, University of Health Sciences, Erzurum, Turkey.
Department of Ophthalmology, Trabzon Kanuni Education and Research Hospital, Health Sciences University, Trabzon, Turkey.
Exp Eye Res. 2025 Jun;255:110387. doi: 10.1016/j.exer.2025.110387. Epub 2025 Apr 9.
This study aimed to investigate biochemically and histopathologically the protective effect of adenosine triphosphate (ATP) and coenzyme Q10 (CoQ10) against potential hydroxychloroquine (HCQ)-induced retinal damage in rats. Twenty-four male albino Wistar-type rats were randomly separated into four groups: healthy (HG), receiving HCQ (HQG), receiving ATP + HCQ (AHQ), and receiving CoQ10 + HCQ (CoQHQ). ATP (4 mg/kg, intraperitoneal) was given to the AHQ, and CoQ10 (10 mg/kg, oral) to the CoQHQ. Rats in the HQG, AHQ, and CoQHQ were given HCQ (120 mg/kg, oral) 1 h after administering ATP and CoQ10. Treatments continued once a day for seven days. On the 8th day, the rats were sacrificed with 50 mg/kg sodium thiopental, and the eyes were removed. Malondialdehyde (MDA), total glutathione (tGSH), superoxide dismutase (SOD), and catalase (CAT), and interleukin-6 (IL-6) levels were measured in the retrieved eye tissues and retinal tissues were assessed histopathologically. An increase in MDA and IL-6 levels and a decrease in tGSH, SOD, and CAT levels were detected in the eye tissues of the HQGcompared to the HG. HCQ-induced changes in oxidant and antioxidant levels were significantly suppressed by ATP and CoQ10 treatment. ATP was more successful than CoQ10 in this inhibition. Severe damage was observed in the eye tissues of the HQG group, whereas the damage was mild in the AHQ and moderate in the CoQHQ. Although both ATP and CoQ10 have the potential to be effective in the prevention of HCQ-induced retinal damage through antioxidative activity, ATP appears to be the more preferable treatment approach.
本研究旨在从生物化学和组织病理学方面研究三磷酸腺苷（ATP）和辅酶Q10（CoQ10）对大鼠潜在的羟氯喹（HCQ）诱导的视网膜损伤的保护作用。将24只雄性白化Wistar型大鼠随机分为四组：健康组（HG）、接受HCQ组（HQG）、接受ATP + HCQ组（AHQ）和接受CoQ10 + HCQ组（CoQHQ）。给AHQ组腹腔注射ATP（4 mg/kg），给CoQHQ组口服CoQ10（10 mg/kg）。在给予ATP和CoQ10 1小时后，给HQG、AHQ和CoQHQ组的大鼠口服HCQ（120 mg/kg）。每天治疗1次，持续7天。在第8天，用50 mg/kg硫喷妥钠处死大鼠，摘除眼球。检测取出的眼组织中丙二醛（MDA）、总谷胱甘肽（tGSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）以及白细胞介素-6（IL-6）的水平，并对视网膜组织进行组织病理学评估。与HG组相比，HQG组眼组织中MDA和IL-6水平升高，tGSH、SOD和CAT水平降低。ATP和CoQ10治疗可显著抑制HCQ诱导的氧化剂和抗氧化剂水平变化。在这种抑制作用中，ATP比CoQ10更有效。HQG组眼组织观察到严重损伤，而AHQ组损伤较轻，CoQHQ组损伤中等。尽管ATP和CoQ10都有可能通过抗氧化活性有效预防HCQ诱导的视网膜损伤，但ATP似乎是更可取的治疗方法。
