Siriwan Wanwisa, Charoenlappanit Sawanya, Phaonakrop Narumon, Thaisakun Siriwan, Roytrakul Sittiruk
Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok, 10900, Thailand.
Functional Proteomics Technology Laboratory, National Center for Genetic and Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand.
Sci Rep. 2025 Apr 12;15(1):12653. doi: 10.1038/s41598-025-97452-y.
Cassava, a major economic crop in Thailand, yielded over 3 million USD in exports in 2023. However, its production has been declining since 2021 due to cassava mosaic disease (CMD) outbreaks, which affect cassava plantations. CMD infections have recently increased due to the scarcity of healthy stems and CMD-resistant varieties, the latter being key to controlling its spread. Developing novel methods is critical for accelerating the cultivation of high-yield, CMD-resistant varieties. In this study, signature peptide patterns were determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and liquid chromatography-tandem MS (LC-MS/MS) to screen for CMD-resistant varieties. Peptide mass fingerprint (PMF) analyses revealed distinct peptide barcodes across 11 varieties, clearly delineating CMD-resistant and CMD-tolerant phenotypes. LC-MS/MS and orthogonal partial least squares-discriminant analysis (OPLS-DA) further demonstrated clear distinctions between the peptide profiles of different phenotypes. Heatmap and PMF analyses consistently revealed unique peptide patterns across the varieties. Volcano plot analysis identified seven upregulated peptides-TATTVAGS, PAAGGGGG, PNELLSYSE, SSIEEGGS, GGGVGGPL, NNGGGFSV, and GPGPAPAA-in CMD-resistant plants. These peptides were associated with proteins containing CONSTANS-like zinc finger, C2H2-type, GST N-terminal, Tubby-like F-box, nuclear-localized AT-hook motif, auxin response factor, and C2 domains. Altogether, this study identified peptidome-based biomarkers for screening CMD-resistant varieties; however, further validation using larger samples is necessary.
木薯是泰国的一种主要经济作物，2023年的出口额超过300万美元。然而，自2021年以来，由于影响木薯种植园的木薯花叶病（CMD）爆发，其产量一直在下降。由于健康茎和抗CMD品种的稀缺，CMD感染最近有所增加，后者是控制其传播的关键。开发新方法对于加速高产、抗CMD品种的培育至关重要。在本研究中，使用基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）和液相色谱-串联质谱（LC-MS/MS）确定特征肽模式，以筛选抗CMD品种。肽质量指纹（PMF）分析揭示了11个品种中不同的肽条形码，清楚地划分了抗CMD和耐CMD的表型。LC-MS/MS和正交偏最小二乘判别分析（OPLS-DA）进一步证明了不同表型的肽谱之间存在明显差异。热图和PMF分析一致揭示了各品种独特的肽模式。火山图分析确定了抗CMD植物中7种上调的肽——TATTVAGS、PAAGGGGG、PNELLSYSE、SSIEEGGS、GGGVGGPL、NNGGGFSV和GPGPAPAA。这些肽与含有CONSTANS样锌指、C2H2型、GST N端、Tubby样F盒、核定位AT钩基序、生长素反应因子和C2结构域的蛋白质相关。总之，本研究确定了基于肽组的生物标志物用于筛选抗CMD品种；然而，需要使用更大的样本进行进一步验证。
