Mejía Karen, Rodríguez-Hernández Adriana-Patricia, Martínez-Hernández Miryam
BioInterphases Laboratory, Division of Graduate Studies and Research, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico.
Laboratory of Molecular Genetics, Division of Graduate Studies and Research, School of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico.
Int J Dent. 2025 Feb 27;2025:1492069. doi: 10.1155/ijod/1492069. eCollection 2025.
Chlorhexidine (CHX) remains the most effective antiseptic in periodontal therapy, multiple reports have identified ultrastructural antibacterial effects of CHX on oral bacteria, however, little is known about its molecular mechanism of action on , an important pathobiont directly associated with the pathogenesis of periodontitis. A standardized suspension of ATCC 33277 was expose to 0.20% CHX for 1 min, then counting colony forming units (CFUs) were recovered to determine the percentage of microbial inhibition. Protein extract integrity of the bacterial cells exposed to CHX was evaluated on a one-dimension sodium dodecyl sulfate polyacrylamide gel electrophoresis (1D SDS-PAGE) gel. The identification of the proteins expressed by after its exposure to CHX was carried out by mass spectrometry (LC-MS). Exposure of for 1 min to 0.20% CHX resulted in a 93% reduction in bacterial viability, in addition to an increase of 2.9-fold in protein expression, with the Lys gingipain protein showing the greatest increase. Exposure to 0.20% CHX 1 min on resulted in 93% reduction in bacterial viability, in addition to inducing changes in the bacterial proteome, with an increased expression of gingipains, the main virulence factor of .
洗必泰（CHX）仍是牙周治疗中最有效的防腐剂。多项报告已证实CHX对口腔细菌具有超微结构抗菌作用，然而，关于其对牙龈卟啉单胞菌（一种与牙周炎发病机制直接相关的重要病理共生菌）的分子作用机制却知之甚少。将标准的牙龈卟啉单胞菌ATCC 33277菌悬液暴露于0.20%的CHX中1分钟，然后回收计数菌落形成单位（CFU）以确定微生物抑制百分比。在一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳（1D SDS-PAGE）凝胶上评估暴露于CHX的细菌细胞的蛋白质提取物完整性。通过质谱（LC-MS）对牙龈卟啉单胞菌暴露于CHX后表达的蛋白质进行鉴定。牙龈卟啉单胞菌暴露于0.20%的CHX 1分钟后，细菌活力降低了93%，此外蛋白质表达增加了2.9倍，其中溶菌酶牙龈蛋白酶蛋白的增加最为显著。牙龈卟啉单胞菌暴露于0.20%的CHX 1分钟后，细菌活力降低了93%，此外还诱导了细菌蛋白质组的变化，牙龈蛋白酶的表达增加，牙龈蛋白酶是牙龈卟啉单胞菌的主要毒力因子。
需注意，原文中部分地方表述不太准确规范，比如“its molecular mechanism of action on, an important pathobiont”这里“on”后面缺少具体对象等，但按照要求进行了完整翻译。
