de Sousa Gabriela Ribeiro, Teles Natanael Ramos de Lima, Silva Carlos Vinicius Azevedo da, Aragão Mariana Costa, Cardoso Domingos Benício Oliveira Silva, Gadelha Francisco Allysson Assis Ferreira, Piuvezam Marcia Regina, Tavares Josean Fechine, Sobral daSilva Marcelo, Barbosa Filho José Maria
Laboratório Multiusuário de Caracterização e Análises, Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos, Centro de Ciências da Saúde, Universidade Federal da Paraíba, João Pessoa 58051-900, Paraíba, Brazil.
Grupo de Pesquisa em Metabolômica e Espectrometria de Massas, Universidade Estadual do Amazonas (UEA), 690065-130, Manaus, Amazonas, Brazil.
ACS Omega. 2025 Mar 27;10(13):13645-13654. doi: 10.1021/acsomega.5c00866. eCollection 2025 Apr 8.
This is the report of the HPLC-MSn-guided isolation of new anti-inflammatory prenylated isoflavonoids and pterocarpans from the stems of using GNPS molecular networking as the main tool. Cluster analysis guided the isolation of five new prenylated isoflavonoids, diffusiflavone A-E ( and ), two prenylated pterocarpans, diffusicarpan A and B ( and ), and two known compounds 6-prenylorobol () and 3--methylquercetin (). The in vitro anti-inflammatory potential of compounds and was assessed in macrophages induced with lipopolysaccharide (LPS). Compounds , , , and were observed to have a reduction in NO levels in at least one of the concentrations tested (1.25, 2.5, 5, 10, and 20 μg/mL) and also reduced IL-1β and IL-6 cytokines, especially diffusicarpan A which reduced cytokine levels in all the concentrations tested.
这是一份关于使用GNPS分子网络作为主要工具，从[植物名称]茎中通过高效液相色谱-质谱联用（HPLC-MSn）引导分离新的抗炎异戊烯基化异黄酮和紫檀烷的报告。聚类分析指导分离出了5种新的异戊烯基化异黄酮，即扩散黄酮A-E（[具体结构]），2种异戊烯基化紫檀烷，扩散紫檀烷A和B（[具体结构]），以及2种已知化合物6-异戊烯基洛罗布醇（[具体结构]）和3'-甲基槲皮素（[具体结构]）。在脂多糖（LPS）诱导的巨噬细胞中评估了化合物[具体化合物]和[具体化合物]的体外抗炎潜力。观察到化合物[具体化合物]、[具体化合物]、[具体化合物]和[具体化合物]在至少一个测试浓度（1.25、2.5、5、10和20μg/mL）下一氧化氮（NO）水平降低，并且还降低了白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）细胞因子，尤其是扩散紫檀烷A在所有测试浓度下均降低了细胞因子水平。
需注意，原文中部分化合物结构及植物名称未给出具体内容，翻译时以[具体结构]、[植物名称]等形式表示。
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