Tâlvan Constantin-Dan, Tâlvan Elena-Teodora, Mohor Călin Ilie, Budișan Liviuța, Grecu Valentin, Mihalache Manuela, Neagoe Ioana Berindan, Zănoagă Oana, Oprinca George Călin, Cristian Adrian Nicolae
Faculty of Medicine, "Lucian Blaga" University of Sibiu, 550169 Sibiu, Romania.
Research Center for Functional Genomic, Biomedicine and Translational Medicine, "Iuliu Hațieganu" University of Medicine and Pharmacy Cluj-Napoca, 400012 Cluj-Napoca, Romania.
Cancers (Basel). 2025 Mar 25;17(7):1091. doi: 10.3390/cancers17071091.
Colorectal cancer (CRC) is a major global health concern with rising incidence and mortality. MicroRNAs (miRNAs) play key roles in tumor progression, invasion, and metastasis. This study aimed to analyze the expression of miR-101-3p, miR-106a-5p, and miR-326 in CRC patients and assess their association with tumor grading and tissue invasion. A total of 40 CRC patients were included in the study. miRNA expression levels were measured using quantitative reverse transcription PCR (qRT-PCR). Statistical analyses, including regression models, were performed to evaluate correlations between miRNA expression, tumor grade, and invasion patterns. MiR-101-3p was significantly downregulated in advanced tumors, while miR-106a-5p expression peaked in Grade 2 CRC. MiR-326 expression was associated with organ metastasis. Regression models confirmed complex interaction patterns among these miRNAs, suggesting their potential role in CRC progression. These findings indicate that miR-101-3p, miR-106a-5p, and miR-326 are associated with CRC severity and invasion. Their expression patterns suggest potential utility as biomarkers for early detection and targeted therapies. Further studies with larger cohorts are needed to validate these results and explore their clinical applications.
结直肠癌（CRC）是一个主要的全球健康问题，其发病率和死亡率不断上升。微小RNA（miRNA）在肿瘤进展、侵袭和转移中起关键作用。本研究旨在分析CRC患者中miR-101-3p、miR-106a-5p和miR-326的表达，并评估它们与肿瘤分级和组织侵袭的关系。共有40例CRC患者纳入本研究。使用定量逆转录PCR（qRT-PCR）测量miRNA表达水平。进行了包括回归模型在内的统计分析，以评估miRNA表达、肿瘤分级和侵袭模式之间的相关性。miR-101-3p在晚期肿瘤中显著下调，而miR-106a-5p的表达在2级CRC中达到峰值。miR-326的表达与器官转移有关。回归模型证实了这些miRNA之间存在复杂的相互作用模式，表明它们在CRC进展中具有潜在作用。这些发现表明，miR-101-3p、miR-106a-5p和miR-326与CRC的严重程度和侵袭有关。它们的表达模式表明，作为早期检测和靶向治疗的生物标志物具有潜在的应用价值。需要更大样本量的进一步研究来验证这些结果并探索其临床应用。
