Shikanai Ayana, Furukawa Satomi, Jiang Sen, Fujimura Satoshi, Kutomi Goro, Saito Mitsue, Tanaka Rica
Division of Regenerative Therapy, Juntendo University Graduates School of Medicine, Tokyo, Japan.
Department of Breast Oncology, Juntendo University School of Medicine, Tokyo, Japan.
Regen Ther. 2025 Mar 29;29:271-281. doi: 10.1016/j.reth.2025.03.018. eCollection 2025 Jun.
Breast reconstruction using fat grafts presents challenges; for example, fat necrosis owing to inadequate blood flow results in reduced engraftment rates. Supplementation of adipose tissue with adipose-derived mesenchymal stem cells (ADSCs) to promote the rapid vascularization of transplanted tissue has been investigated. However, the vascularization of fat-grafted tissues using only ADSC transplantation is limited. cultured mononuclear cells (RE-01) are a cell population with highly vascular and tissue-regenerative properties. This study aimed to evaluate the effect of combining RE-01 cells and ADSCs on the engraftment rate of fat grafts and explore the potential of this approach as a new option for breast reconstruction surgery. We hypothesized that combining RE-01 with ADSCs might promote angiogenesis and improve the fat grafting rate, consequently reducing the number of ADSCs required.
ADSCs cultured from human adipose tissue discarded during liposuction were co-cultured with RE-01 cells produced from the peripheral blood of healthy volunteers. vascular regeneration and adipogenic differentiation potential were analyzed. In addition, fat grafting experiments were conducted using nude mice to verify the fat grafting efficacy of ADSCs after co-cultivation with RE-01.
ADSCs co-cultured with RE-01 cells promoted angiogenesis and adipogenesis . This was evidenced by a significant increase in the expression of adipogenic markers and , as well as enhanced lipid droplet formation observed through Oil Red O staining. The results demonstrated that the fat engraftment rate was significantly improved in the mixed group of ADSCs co-cultured with RE-01 cells. The number of blood vessels and fat quality of the transplanted adipose tissue were also increased in this group, suggesting that ADSCs co-cultured with RE-01 cells were highly effective in fat transplantation.
ADSCs co-cultured with RE-01 cells may be useful for improving the engraftment rate of fat grafts. However, further studies are required to verify the mechanisms.
使用脂肪移植进行乳房重建存在挑战；例如，由于血流不足导致的脂肪坏死会降低移植率。已研究通过补充脂肪来源的间充质干细胞（ADSCs）来促进移植组织的快速血管化。然而，仅使用ADSC移植的脂肪移植组织的血管化是有限的。培养的单核细胞（RE-01）是具有高度血管生成和组织再生特性的细胞群体。本研究旨在评估RE-01细胞与ADSCs联合使用对脂肪移植成活率的影响，并探索这种方法作为乳房重建手术新选择的潜力。我们假设将RE-01与ADSCs联合使用可能会促进血管生成并提高脂肪移植率，从而减少所需的ADSCs数量。
从抽脂术中丢弃的人体脂肪组织中培养的ADSCs与健康志愿者外周血产生的RE-01细胞共培养。分析血管再生和成脂分化潜力。此外，使用裸鼠进行脂肪移植实验，以验证与RE-01共培养后ADSCs的脂肪移植效果。
与RE-01细胞共培养的ADSCs促进了血管生成和成脂作用。这通过成脂标志物和的表达显著增加以及通过油红O染色观察到的脂滴形成增强得到证明。结果表明，与RE-01细胞共培养的ADSCs混合组的脂肪移植率显著提高。该组移植脂肪组织的血管数量和脂肪质量也有所增加，表明与RE-01细胞共培养的ADSCs在脂肪移植中非常有效。
与RE-01细胞共培养的ADSCs可能有助于提高脂肪移植的成活率。然而，需要进一步研究来验证其机制。
