Tang Jieqiong, Tudi Xierenayi, Zhang Tianxiang, Zhu Jingbo, Shen Tongtong
Department of Cardiology, Chuzhou Hospital Affiliated to Anhui Medical University, Chuzhou, China.
Beijing Institute of Heart, Lung and Blood Vessel Diseases, Beijing Anzhen Hospital, Capital Medical University, Beijing, China.
Front Cardiovasc Med. 2025 Mar 31;12:1516043. doi: 10.3389/fcvm.2025.1516043. eCollection 2025.
Myocardial infarction (MI) is a leading cause of death worldwide. Immune cells play a significant role in the MI development. This study aims to identify a marker related to neutrophil for the diagnosis and early progression of MI.
Key genes were screened using three machine learning algorithms to establish a diagnostic model. A gene associated with the early progression of MI was identified based on single cell RNA sequencing data. To further validate the predictive value of the gene, the mouse models of MI were constructed. Immunofluorescence (IF) analysis demonstrated the co-expression of the gene with neutrophils. Immunohistochemistry (IHC) was performed to validate the role of the gene in the progression of MI.
Neutrophils were identified and verified as the key infiltrating immune cells (IICs) involved in the onset of MI. A diagnostic panel with superior performance was developed using five key genes related to neutrophils in MI (AUC = 0.887). Among the panel, IL1R2 was found to early phase of MI, which was further corroborated by IHC in mouse models of MI.
This study suggests that IL1R2, which is specific to neutrophils, can predict the diagnosis and early progression of MI, providing new insights into the clinical management of MI.
心肌梗死（MI）是全球主要的死亡原因之一。免疫细胞在心肌梗死的发展中起着重要作用。本研究旨在确定一种与中性粒细胞相关的标志物，用于心肌梗死的诊断和早期进展评估。
使用三种机器学习算法筛选关键基因，以建立诊断模型。基于单细胞RNA测序数据确定与心肌梗死早期进展相关的基因。为进一步验证该基因的预测价值，构建了心肌梗死小鼠模型。免疫荧光（IF）分析显示该基因与中性粒细胞共表达。进行免疫组织化学（IHC）以验证该基因在心肌梗死进展中的作用。
中性粒细胞被鉴定并确认为参与心肌梗死发病的关键浸润免疫细胞（IICs）。利用与心肌梗死中中性粒细胞相关的五个关键基因开发了一个性能优越的诊断面板（AUC = 0.887）。在该面板中，发现IL1R2与心肌梗死的早期阶段相关，这在心肌梗死小鼠模型的免疫组织化学中得到了进一步证实。
本研究表明，中性粒细胞特异性的IL1R2可以预测心肌梗死的诊断和早期进展，为心肌梗死的临床管理提供了新的见解。
