羟基自由基（HO）对用生长素和壳梭孢菌素处理的玉米胚芽鞘细胞伸长生长和氧化应激的影响。

The effect of HO on elongation growth and oxidative stress in maize coleoptile cells treated with auxin and fusicoccin.

作者信息

Siemieniuk Agnieszka, Rudnicka Małgorzata, Karcz Waldemar

机构信息

Plant Ecophysiology Team, Faculty of Natural Sciences, Institute of Biology, Biotechnology and Environmental Protection, University of Silesia in Katowice, 28 Jagiellońska St., 40-032, Katowice, Poland.

出版信息

Sci Rep. 2025 Apr 15;15(1):12903. doi: 10.1038/s41598-025-96912-9.

DOI:10.1038/s41598-025-96912-9

PMID:40234506

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12000566/

Abstract

The aim of this study was to evaluate the impact of hydrogen peroxide (HO) on the elongation growth of maize coleoptile cells induced by auxin (IAA) and fusicoccin (FC) according to the "acid growth theory". The key component of this process is PM H-ATPase activity and the resulting proton extrusion. In order to complete this objective, measurements of coleoptile growth were made, pump activity was analyzed through changes in environmental pH and cell membrane potential, and the impact on oxidative stress in response to HO was determined. It was found that although hydrogen peroxide restricts acid growth induced by both IAA and FC to a similar level, the PM H-ATPase activity is inhibited differently. These findings indicate that in the presence of HO, the previously described wall-stiffening process might be the primary limiting factor in the elongation growth of maize coleoptile cells.

摘要

本研究的目的是根据“酸生长理论”，评估过氧化氢（HO）对生长素（IAA）和壳梭孢菌素（FC）诱导的玉米胚芽鞘细胞伸长生长的影响。该过程的关键组成部分是质膜H⁺-ATP酶活性以及由此产生的质子外排。为了实现这一目标，对胚芽鞘生长进行了测量，通过环境pH值和细胞膜电位的变化分析了质子泵活性，并确定了HO对氧化应激的影响。结果发现，尽管过氧化氢将IAA和FC诱导的酸生长限制在相似水平，但质膜H⁺-ATP酶活性受到的抑制有所不同。这些发现表明，在HO存在的情况下，先前描述的细胞壁硬化过程可能是玉米胚芽鞘细胞伸长生长的主要限制因素。