Tsuchida Y, Yokota S, Bessho F, Yokomori K, Makino S, Saito S
Tumour Biol. 1985;6(1):67-73.
Neuron-specific enolase (gamma-subunit isozyme) in extracts of human neuroblastomas and other tumors in children was measured by means of a radio-immunoassay in order to elucidate the rationale for clinical determination of its serum concentrations in patients. Surgical specimens as well as tumors xenotransplanted in nude mice were studied. The neuron-specific enolase (NSE) concentrations in extracts of xenografted and surgical neuroblastomas were 1,733.2 +/- 677.3 (range; 883-2,659) ng/mg protein and 1,735.3 +/- 941.1 (559-2,933) ng/mg protein, respectively, while other pediatric tumors examined showed much lower levels of NSE: 121.5 +/- 146.1 (22-374) ng/mg protein in xenografts and 17.7-610 ng/mg protein in surgical specimens. Sera of nude mice bearing human neuroblastomas also showed NSE concentrations ranging from 1,102 to 4,400 ng/ml, very much higher than those in sera of nude mice without tumors or with other pediatric tumors. These results tend to confirm the specificity of NSE for neuroblastoma, and the difference in NSE concentrations among five neuroblastoma xenografts is discussed in relation to their cytogenetic characteristics.
为阐明临床上测定儿童患者血清神经元特异性烯醇化酶（γ亚基同工酶）浓度的原理，采用放射免疫分析法对人神经母细胞瘤及其他儿童肿瘤提取物中的该酶进行了测定。研究了手术标本以及移植到裸鼠体内的异种移植肿瘤。异种移植和手术切除的神经母细胞瘤提取物中神经元特异性烯醇化酶（NSE）浓度分别为1,733.2±677.3（范围：883 - 2,659）ng/mg蛋白和1,735.3±941.1（559 - 2,933）ng/mg蛋白，而所检测的其他儿科肿瘤的NSE水平则低得多：异种移植瘤中为121.5±146.1（22 - 374）ng/mg蛋白，手术标本中为17.7 - 610 ng/mg蛋白。携带人神经母细胞瘤的裸鼠血清中NSE浓度也在1,102至4,400 ng/ml之间，远高于无肿瘤或患有其他儿科肿瘤的裸鼠血清中的浓度。这些结果倾向于证实NSE对神经母细胞瘤的特异性，并结合其细胞遗传学特征讨论了五种神经母细胞瘤异种移植瘤中NSE浓度的差异。
