酸中毒增强了钙激活氯离子通道在出生后早期对血管张力调节的作用。
Acidosis enhances contribution of Ca-activated chloride channels to vascular tone regulation in early postnatal period.
作者信息
Shvetsova Anastasia A, Khlystova Margarita A, Shateeva Valentina S, Simonenko Sofia D, Borzykh Anna A, Abramochkin Denis V, Gaynullina Dina K
机构信息
Lomonosov Moscow State University, Moscow, Russia.
Institute of Biomedical Problems, Russian Academy of Sciences, Moscow, Russia.
出版信息
Curr Res Physiol. 2025 Mar 28;8:100143. doi: 10.1016/j.crphys.2025.100143. eCollection 2025.
INTRODUCTION
Acidosis often occurs during clinical complications in newborns and can lead to changes in the mechanisms of arterial tone regulation. However, it is unknown how acidosis affects the activity of Ca-activated chloride channels (CaCC) in arteries during early ontogenesis. We hypothesized that their activity may increase during acidosis.
METHODS
We studied isometric contractions of saphenous arteries isolated from adult and 10-13-day-old rats. Intracellular pH was measured using a fluorescent indicator BCECF-AM simultaneously with recording the contractile activity of the arterial preparation in isometric mode.
RESULTS
Metabolic acidosis with pH = 6.8 caused a significant decrease in the arterial contractile responses of adult and 10-13-day-old rats. The functional contribution of CaCC was absent in the adult rat arteries both at pH = 7.4 and pH = 6.8. However, in 10-13-day-old rat pups, the functional contribution of CaCC was higher at pH = 6.8 compared to pH = 7.4.
CONCLUSION
Acidosis augments the functional role of CaCC in arteries during early postnatal ontogenesis, but not in adulthood.
引言
酸中毒在新生儿临床并发症期间经常发生，并可导致动脉张力调节机制的改变。然而，在早期个体发育过程中，酸中毒如何影响动脉中钙激活氯离子通道（CaCC）的活性尚不清楚。我们推测在酸中毒期间它们的活性可能会增加。
方法
我们研究了从成年大鼠和10 - 13日龄大鼠分离的隐动脉的等长收缩。使用荧光指示剂BCECF - AM测量细胞内pH值，同时以等长模式记录动脉制剂的收缩活性。
结果
pH = 6.8的代谢性酸中毒导致成年大鼠和10 - 13日龄大鼠的动脉收缩反应显著降低。在pH = 7.4和pH = 6.8时，成年大鼠动脉中CaCC的功能贡献均不存在。然而，在10 - 13日龄的幼鼠中，与pH = 7.4相比，pH = 6.8时CaCC的功能贡献更高。
结论
酸中毒在出生后早期个体发育过程中增强了CaCC在动脉中的功能作用，但在成年期则不然。
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