谷胱甘肽过氧化物酶4基因3'UTR区rs713041单核苷酸多态性与口腔癌前病变易感性的相关性分析

Single nucleotide polymorphism analysis of 3'UTR rs713041 of Glutathione peroxidase 4 association with susceptibility to oral premalignant disorders.

作者信息

Subbiah Usha, Sidhic Nihala

机构信息

Human Genetics Research Centre, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, Pallikaranai Chennai, Tamil Nadu, 600 100, India.

出版信息

Oral Maxillofac Surg. 2025 Apr 21;29(1):89. doi: 10.1007/s10006-025-01380-0.

DOI:10.1007/s10006-025-01380-0

PMID:40257589

Abstract

PURPOSE

Oral potentially malignant disorders (OPMDs) affect the oral mucosa and increase the risk of oral cancer. Glutathione peroxidase 4 (GPX4), a key antioxidant mediator and regulator of ferroptosis, has garnered significant attention in cancer research. This study is to investigate the association between the single nucleotide polymorphism GPX4 3'UTR rs713041 and oral premalignancy disorders.

METHODS

The rs713041of GPX4 were analysed using PCR-RFLP IN 600 subjects including OSMF, leukoplakia and healthy controls along with their habitual factors.

RESULTS

Chewing and smoking habits were present in 52% and 69% of OSMF cases, and 62% and 57% of leukoplakia cases, respectively. Disease prevalence was 78% in males and 59% in females for OSMF, and 22% in males and 26% in females for leukoplakia. The allele frequency distribution for OSMF did not significantly deviate from Hardy-Weinberg equilibrium. The heterozygous TC genotype in OSMF showed a significant association with an odds ratio of 2.42 (CI: 1.58-3.72, P = 0.00) compared to controls.

CONCLUSION

The GPX4 3'UTR T/C carrier genotype is associated with an increased risk of OSMF and leukoplakia. This genotype could serve as a predictive marker for the risk of oral premalignancy disorders.

CLINICAL TRAIL NUMBER

Not applicable.

摘要

目的

口腔潜在恶性疾病（OPMDs）会影响口腔黏膜并增加患口腔癌的风险。谷胱甘肽过氧化物酶4（GPX4）是铁死亡的关键抗氧化介质和调节因子，在癌症研究中受到了广泛关注。本研究旨在探讨单核苷酸多态性GPX4 3'UTR rs713041与口腔癌前病变之间的关联。

方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析600名受试者（包括口腔黏膜下纤维化患者、白斑患者和健康对照）的GPX4 rs713041，同时分析他们的习惯因素。

结果

口腔黏膜下纤维化患者中咀嚼和吸烟习惯的比例分别为52%和69%，白斑患者中分别为62%和57%。口腔黏膜下纤维化男性患病率为78%，女性为59%；白斑男性患病率为22%，女性为26%。口腔黏膜下纤维化的等位基因频率分布未显著偏离哈迪-温伯格平衡。与对照组相比，口腔黏膜下纤维化患者的杂合子TC基因型显示出显著关联，比值比为2.42（置信区间：1.58-3.72，P = 0.00）。