Barth Albert, Perry Viv E A, Hamilton Lauren E, Sutovsky Peter, Oko Richard
Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Queensland Sperm Morphology Laboratory (QSML), Goondiwindi, QLD, Australia.
Adv Anat Embryol Cell Biol. 2025;240:165-202. doi: 10.1007/978-3-031-70126-9_4.
This chapter is intended to provide an accurate visual description of normal and abnormal bovine sperm morphology as seen under the light microscope. The photographs were taken × 1000 magnification and enlarged × 1.6, unless specified otherwise, using either a) differential interference microscopy (DIC), b) phase contrast microscopy, c) eosin-nigrosin-stained sperm smears, or d) Feulgen stained smears as indicated in the legends.When viewed with a critical eye, there is an extremely wide variation in form and structure within the various categories of sperm cell abnormalities. It would be an impossible and fruitless task to attempt to show every minor variation in appearance of each type. Therefore, a series of photographs has been put together showing the main features of each type of abnormality so that any other deviation observed can easily be recognized as to the category in which it belongs.The nomenclature applied to the various abnormalities is that preferred by us, although generally accepted by other workers. Synonymous nomenclature used by other workers is included in the discussion on interpretation of sperm cell abnormalities in Chapter 5 .
本章旨在对在光学显微镜下观察到的正常和异常牛精子形态提供准确的视觉描述。除非另有说明,照片均在×1000倍放大倍数下拍摄,并放大1.6倍,使用以下方法之一:a)微分干涉显微镜(DIC),b)相差显微镜,c)伊红 - 黑色素染色精子涂片,或d)如图例所示的福尔根染色涂片。当以挑剔的眼光观察时,各类精子细胞异常在形态和结构上存在极大差异。试图展示每种类型外观上的每一个微小变化将是一项不可能且徒劳的任务。因此,已汇集了一系列照片,展示每种异常类型的主要特征,以便任何观察到的其他偏差都能轻松被识别出所属类别。应用于各种异常的命名法是我们所偏好的,尽管其他研究者也普遍接受。其他研究者使用的同义命名法包含在第5章关于精子细胞异常解释的讨论中。