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[源自人肾细胞癌的细胞系的特性]

[Characteristics of a cell line obtained from a human hypernephroma].

作者信息

Shevliagin V Ia, Shaposhnikova G M, Snegireva A E, Chizhevskaia V I, Miller G G

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1985(6):29-33.

PMID:4027291
Abstract

A stable tumorigenic cell line has been obtained from the human hypernephrome. The culture grew as monolayers of large polygonal cells with foamy cytoplasm and 1-2 nucleoli. The cells formed monolayer after the third day after planting into flask and then formed multilayered growth. The electron microscopic studies of hypernephrome sections revealed the dark and light cells. The hypernephrome cells have characteristics of tumor cells. On the 200th passage the cells are aneuploid with a model number of 62 chromosomes; an acrocentric chromosome in D group is noted as a marker one. The hypernephrome cells are free of Mycoplasma contamination, able to support the several virus replication.

摘要

已从人类肾上腺皮质癌中获得了一种稳定的致瘤细胞系。培养物以具有泡沫状细胞质和1 - 2个核仁的大的多边形细胞单层生长。接种到培养瓶中第三天后细胞形成单层,然后形成多层生长。肾上腺皮质癌切片的电子显微镜研究显示有深色和浅色细胞。肾上腺皮质癌细胞具有肿瘤细胞的特征。在第200代时,细胞为非整倍体,染色体模式数为62条;D组中的一条近端着丝粒染色体被视为标记染色体。肾上腺皮质癌细胞无支原体污染,能够支持多种病毒复制。

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