Wang Xiaomeng, Jia Xia, Zhao Yonghua, Xie Yuan, Meng Xiuxin, Wang Fang
Key Laboratory of Subsurface Hydrology and Ecological Effects in Arid Region of Ministry of Education, Shaanxi Key Laboratory of Land Consolidation, Key Laboratory of Eco-Hydrology and Water Security in Arid and Semi-Arid Regions of Ministry of Water Resources, School of Water and Environment, Chang'an University, Xi'an 710054, China.
School of Land Engineering, Chang'an University, Xi'an 710054, China.
Biology (Basel). 2025 Mar 31;14(4):362. doi: 10.3390/biology14040362.
(1) Background: Rhizobia can promote plant growth by providing essential nutrients such as NH and PO; thus, rhizobia that can tolerate the stress of heavy metals will be conducive to the phytoremediation of heavy-metal-contaminated soils. Therefore, understanding the dominant heavy-metal-tolerant rhizobia that can be cultured is important for the establishment of an indigenous legume-rhizobia symbiotic remediation system; (2) Methods: Here, we investigated gene diversity in culturable rhizobia from black locust ( L.) grown in cadmium (Cd)-contaminated soils using high-throughput sequencing.; (3) Results: A total of 16 genera and 26 species were identified from the cultures of root nodules of black locust exposed to five Cd levels. Cadmium did not show a significant effect on the abundance, diversity, and evenness of the culturable rhizobia community. However, Cd significantly affected the community structure of culturable rhizobia containing , and were the absolute dominant genera present in the cultures under five Cd treatments. Additionally, Cd significantly affected the relative abundance of and in the cultures. Soil pH, total Cd, DTPA-Cd, and C/H ratio were the significant factors on culturable rhizobia community.; (4) Conclusions: Cd showed a negative effect on gene community of culturable rhizobia from black locust, which will provide insight into the selection of excellent strains that can promote phytoremediation of heavy-metal-contaminated soils.
(1) 背景：根瘤菌可通过提供氨和磷酸盐等必需养分促进植物生长；因此，能够耐受重金属胁迫的根瘤菌将有助于对重金属污染土壤进行植物修复。所以，了解可培养的优势重金属耐受根瘤菌对于建立本土豆科植物 - 根瘤菌共生修复系统很重要；(2) 方法：在此，我们使用高通量测序研究了生长在镉（Cd）污染土壤中的刺槐（Robinia pseudoacacia L.）可培养根瘤菌中的基因多样性。；(3) 结果：从暴露于五个镉水平的刺槐根瘤培养物中鉴定出总共16个属和26个种。镉对可培养根瘤菌群落的丰度、多样性和均匀度未显示出显著影响。然而，镉显著影响了含有[具体属名未给出]的可培养根瘤菌的群落结构，并且[具体属名未给出]是五种镉处理下培养物中存在的绝对优势属。此外，镉显著影响了培养物中[具体属名未给出]和[具体属名未给出]的相对丰度。土壤pH、总镉、DTPA - 镉和碳/氢比是影响可培养根瘤菌群落的显著因素。；(4) 结论：镉对刺槐可培养根瘤菌的[具体基因未给出]基因群落有负面影响，这将为选择能够促进重金属污染土壤植物修复的优良菌株提供见解。
