Chen Jun-Lv, Sun Sheng-Ren, Wang Zhu-Qing, Fu Hua-Ying, Xu Huan-Yin, Chang Hai-Long, Gao San-Ji, Wang Qin-Nan
Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 510000, China.
National Key Laboratory for Tropical Crop Breeding, Haikou 571101, China.
Plants (Basel). 2025 Apr 16;14(8):1221. doi: 10.3390/plants14081221.
Sugarcane rust diseases are caused by (brown rust) and (orange rust), and significantly threaten the sustainable and stable development of the global sugarcane industry. within the complex is a potential germplasm resource for sugarcane breeding and is characterized by its tolerance of infertile land, drought, and diseases. However, the research on resistance to rust in clones and their offspring (F1 and backcross with modern sugarcane varieties) is limited. In this study, a total of 201 leaf samples from spp. hybrids with rust symptoms were collected and screened for disease occurrence. PCR detection revealed that 17.9% and 34.8% of the samples were infected by and , respectively. Additionally, 12.9% of the samples were infected by both pathogens. A total of 88 clones of offspring and the parents plus 3 additional were selected for the identification of brown rust resistance by an artificial inoculation method. Among them, 61 clones displayed high resistance to brown rust. Molecular detection showed that 13 offspring of and 6 backcross parents of the "ROC" series exhibited the major resistance gene () for brown rust. Unexpectedly, the gene was absent in 42 clones that were resistant to brown rust, suggesting that other resistance genes for brown rust likely exist in and their offspring. Our results offer some significant genetic resources for developing sugarcane cultivars with resistance against rust.
甘蔗锈病由（褐锈病）和（橙锈病）引起，严重威胁全球甘蔗产业的可持续稳定发展。甘蔗属复合体中的某一品种是甘蔗育种的潜在种质资源，其特点是耐贫瘠土地、耐旱且抗病。然而，关于该品种无性系及其后代（F1代以及与现代甘蔗品种的回交后代）对锈病抗性的研究有限。在本研究中，共收集了201份来自具有锈病症状的甘蔗属杂种的叶片样本，并对病害发生情况进行筛选。PCR检测显示，分别有17.9%和34.8%的样本感染了和。此外，12.9%的样本同时感染了这两种病原菌。总共选择了88个该品种后代无性系及其亲本，外加3个其他品种，通过人工接种法鉴定对褐锈病的抗性。其中，61个无性系对褐锈病表现出高抗性。分子检测表明，该品种的13个后代以及“ROC”系列的6个回交亲本表现出对褐锈病的主要抗性基因（）。出乎意料的是，42个对褐锈病具有抗性的无性系中不存在该基因，这表明甘蔗属及其后代可能存在其他抗褐锈病的基因。我们的研究结果为培育抗锈病甘蔗品种提供了一些重要的遗传资源。
