Jiang Yu, He Pengfei, Kong Huihui, He Pengbo, Wu Yixin, Tang Guowen, Tang Ping, Di Yining, Li Xingyu, Liu Lufeng, Munir Shahzad, He Yueqiu
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
Microorganisms. 2025 Mar 28;13(4):770. doi: 10.3390/microorganisms13040770.
Potato common scab, caused by species, is a widespread soil-borne disease that poses a significant threat to potato cultivation globally. In this study, a D7-8 strain was isolated from a potato. This endophytic bacterium exhibited broad-spectrum antifungal activity, and pot trials demonstrated that the D7-8 strain effectively controlled potato common scab with an efficacy of 42.07%. The complete genome sequence of the D7-8 strain was sequenced and subsequently identified as through multiple bioinformatic methods, primarily through structural variation analysis of whole-genome sequences. The machine learning method predicted that the expression profiles of colinear genes among closely related species were highly consistent. Metabolite analysis of crude extracts using ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive HRMS) revealed that D7-8 produces bioactive compounds, including surfactin and fengycin, both known for their antimicrobial properties. This study elucidates the antagonistic effect of D7-8 against and provides a valuable reference for future research on accurate microbial identification.
马铃薯普通疮痂病由多种病原菌引起，是一种广泛传播的土传病害，对全球马铃薯种植构成重大威胁。在本研究中，从马铃薯中分离出一株D7 - 8菌株。这种内生细菌表现出广谱抗真菌活性，盆栽试验表明D7 - 8菌株能有效防治马铃薯普通疮痂病，防治效果为42.07%。对D7 - 8菌株的全基因组序列进行了测序，并通过多种生物信息学方法，主要是通过全基因组序列的结构变异分析，随后将其鉴定为[具体菌种]。机器学习方法预测，密切相关的[相关菌种]中线性基因的表达谱高度一致。使用超高效液相色谱与四极杆 - 轨道阱高分辨率质谱联用（UPLC - Q - Exactive HRMS）对粗提物进行代谢物分析，结果表明D7 - 8产生生物活性化合物，包括具有抗菌特性的表面活性素和丰原素。本研究阐明了D7 - 8对[病原菌]的拮抗作用，并为未来准确的微生物鉴定研究提供了有价值的参考。
