An Hui, Zhao Yuhan, Deng Xiaohong, Hu Wei, Zhang Xia, Zheng Shuo, Yao Longshuai, Meng Fanliang, Fang Zheng, Xu Fanghua, Qiu Jianhua, Li Ning, Wang Gang
Shandong Provincial Key Laboratory of Zoonoses, College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China.
Zhejiang ECO-BIOK Animal Health, Shanghai 200063, China.
Vet Sci. 2025 Apr 9;12(4):348. doi: 10.3390/vetsci12040348.
Porcine reproductive and respiratory syndrome (PRRS) is one of the most serious diseases threatening the swine industry worldwide. However, no satisfactory control strategy has existed until now. In this study, the effectiveness of tylvalosin against PRRSV and the underlying mechanism was investigated. The results showed that in porcine alveolar macrophages (PAMs), tylvalosin can inhibit the replication of the NADC30-like and NADC34-like strains in a dose-dependent manner. It is worth noting that pre-incubation with tylvalosin had no significant inhibitory effect on the NADC30-like strain but did inhibit the NADC34-like strain. Co-incubation of both viruses and tylvalosin or post-incubation with tylvalosin after viral infection inhibited PRRSV. We further analyzed the effect of tylvalosin on different stages of PRRSV replication and found that the stages in the PRRSV life cycle could be blocked by tylvalosin. Tylvalosin has an antiviral effect on all four stages of the NADC34-like strain's infectious cycle but has no effect against the adsorption phase of the NADC30-like strain. These results demonstrated that tylvalosin suppressed PRRSV infection in PAMs and inhibited PRRSV infection at multiple steps of the viral life cycle. This study will contribute to the clinical prevention and control of PRRS and provide a basis for further exploration of the anti-PRRSV effects of tylvalosin.
猪繁殖与呼吸综合征（PRRS）是威胁全球养猪业的最严重疾病之一。然而，迄今为止尚无令人满意的防控策略。在本研究中，调查了泰万菌素对猪繁殖与呼吸综合征病毒（PRRSV）的有效性及其潜在机制。结果表明，在猪肺泡巨噬细胞（PAMs）中，泰万菌素能以剂量依赖的方式抑制NADC30样和NADC34样毒株的复制。值得注意的是，预先用泰万菌素孵育对NADC30样毒株没有显著抑制作用，但对NADC34样毒株有抑制作用。病毒与泰万菌素共同孵育或病毒感染后用泰万菌素后孵育均可抑制PRRSV。我们进一步分析了泰万菌素对PRRSV复制不同阶段的影响，发现PRRSV生命周期中的各个阶段均可被泰万菌素阻断。泰万菌素对NADC34样毒株感染周期的所有四个阶段均有抗病毒作用，但对NADC30样毒株的吸附阶段无作用。这些结果表明，泰万菌素可抑制PAMs中PRRSV的感染，并在病毒生命周期的多个步骤抑制PRRSV感染。本研究将有助于PRRS的临床防控，并为进一步探索泰万菌素的抗PRRSV作用提供依据。
