Chu Yi, Chen Na, Liu Yuting, Zhou Lianghong, Zhang Jinghui, Zhao Jingwu, Zhao Yaxiang, Li Zhe, Yang Su, Chen Xiaodong
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Animal Science and Technology &College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Hubei Yidanyuan Agricultural and Animal Husbandry Technology Co. LTD, Yingcheng, China.
Mol Nutr Food Res. 2025 Jun;69(12):e70082. doi: 10.1002/mnfr.70082. Epub 2025 Apr 26.
This study aimed to utilize aged laying hens as a model to investigate the effects of naringin on the occurrence and progression of metabolic dysfunction-associated fatty liver disease (MAFLD), along with its underlying regulatory mechanisms. A total of 288 aged laying hens, 50-week-old, were divided into four groups: a normal diet (ND) group, and three naringin groups receiving 200 mg/kg (N1), 400 mg/kg (N2), and 600 mg/kg (N3). The experiment lasted for 10 weeks, after which serum, liver, and cecal contents were collected from the hens. Results indicated that dietary naringin supplementation reduced hepatic lipid deposition, lowered blood lipid levels, improved antioxidant capacity, and promoted estradiol secretion. Additionally, 16S rDNA analysis revealed that naringin enhanced microbial diversity in the cecum and regulated the abundance of gut microbes associated with fatty liver. Untargeted metabolomics of blood demonstrated that naringin decreased the concentration of glycerophospholipid and sterol lipid metabolites while increasing levels of pantothenic acids and amino acid metabolites. Furthermore, liver transcriptome analysis indicated that naringin interfered with fatty acid synthesis and transport processes while enhancing fatty acid oxidation. Dietary naringin supplementation can mitigate the occurrence of MAFLD by regulating the gut-liver axis and estrogen signaling, particularly in postmenopausal women.
本研究旨在利用老龄蛋鸡作为模型，探讨柚皮苷对代谢功能障碍相关脂肪性肝病（MAFLD）发生发展的影响及其潜在调控机制。选取288只50周龄的老龄蛋鸡，分为四组：正常饮食（ND）组，以及三个分别给予200 mg/kg（N1）、400 mg/kg（N2）和600 mg/kg（N3）柚皮苷的组。实验持续10周，之后收集母鸡的血清、肝脏和盲肠内容物。结果表明，日粮中添加柚皮苷可减少肝脏脂质沉积，降低血脂水平，提高抗氧化能力，并促进雌二醇分泌。此外，16S rDNA分析显示，柚皮苷可增强盲肠微生物多样性，并调节与脂肪肝相关的肠道微生物丰度。血液非靶向代谢组学表明，柚皮苷可降低甘油磷脂和甾醇脂质代谢物的浓度，同时提高泛酸和氨基酸代谢物的水平。此外，肝脏转录组分析表明，柚皮苷可干扰脂肪酸合成和转运过程，同时增强脂肪酸氧化。日粮中添加柚皮苷可通过调节肠-肝轴和雌激素信号减轻MAFLD的发生，尤其是在绝经后女性中。
