Ramezaniaghdam Maryam, Bohlender Lennard L, Parsons Juliana, Hoernstein Sebastian N W, Decker Eva L, Reski Ralf
Plant Biotechnology, Faculty of Biology, University of Freiburg, Schaenzlestr. 1, 79104, Freiburg, Germany.
Cluster of Excellence livMatS @ FIT-Freiburg Centre for Interactive Materials and Bioinspired Technologies, University of Freiburg, Georges-Köhler-Allee 105, 79110, Freiburg, Germany.
Plant Cell Rep. 2025 Apr 26;44(5):103. doi: 10.1007/s00299-025-03485-y.
We report the successful moss-produced recombinant spider silk key protein component containing both the N- and the C-terminal domain. Spider dragline silk stands out as a remarkable biomaterial, representing one of nature's toughest fibres. Its strength rivals that of many synthetic fibres used commercially, rendering it applicable across various industrial and medical domains. However, its widespread utilisation requires cost-effective mass production. Biotechnology presents a promising avenue for achieving this goal, particularly through the production of recombinant dragline silk proteins in transgenic plant systems. This study aimed to assess the feasibility of producing one key protein component of dragline silk, MaSp1, from the western black widow spider, Latrodectus hesperus, the protein LhMaSp1, in the moss Physcomitrella (Physcomitrium patens). Here, we present the successful recombinant production of spider silk protein containing both the N- and C-terminal domains of LhMaSp1 in moss cells. The production of recombinant LhMaSp1 protein in Physcomitrella was performed in shake flasks and in five-litre photobioreactors and the correct synthesis of LhMaSp1 was proven via mass spectrometry. We estimate that the yield of recombinant spider silk protein in Physcomitrella bioreactors is above 0.82 mg/g fresh weight.
我们报道了在苔藓中成功生产出包含N端和C端结构域的重组蜘蛛丝关键蛋白成分。蜘蛛的拖牵丝是一种卓越的生物材料，是自然界中最坚韧的纤维之一。其强度可与许多商业使用的合成纤维相媲美，使其适用于各种工业和医学领域。然而，其广泛应用需要具有成本效益的大规模生产。生物技术为实现这一目标提供了一条有前景的途径，特别是通过在转基因植物系统中生产重组拖牵丝蛋白。本研究旨在评估在苔藓小立碗藓（Physcomitrium patens）中生产西方黑寡妇蜘蛛（Latrodectus hesperus）拖牵丝的一种关键蛋白成分——蛋白LhMaSp1的可行性。在此，我们展示了在苔藓细胞中成功重组生产出包含LhMaSp1的N端和C端结构域的蜘蛛丝蛋白。在摇瓶和五升光生物反应器中进行了小立碗藓中重组LhMaSp1蛋白的生产，并通过质谱法证明了LhMaSp1的正确合成。我们估计小立碗藓生物反应器中重组蜘蛛丝蛋白的产量高于0.82毫克/克鲜重。
