Belloir Christine, Jeannin Mathilde, Karolkowski Adeline, Briand Loïc
Centre des Sciences du Goût et de l'Alimentation, The National Centre for Scientific Research (CNRS), National Institute of Agricultural Research (INRAE), Institut Agro, Université Bourgogne Europe, F-21000 Dijon, France.
Nutrients. 2025 Mar 8;17(6):949. doi: 10.3390/nu17060949.
BACKGROUND/OBJECTIVES: Studies have hypothesised that single-nucleotide polymorphisms (SNPs) in the and genes may alter sweet compound detection and eating habits, thereby increasing the risk of obesity. This in vitro study aims to measure the impact of human / polymorphisms, some of which are thought to be involved in obesity, on the response of the sweet taste receptor to various sweeteners. It also aims to identify new SNPs in an obese population associated with a decrease in or loss of function.
First, the effects of 12 human -SNPs and 16 human -SNPs, previously identified in the literature, on the response of the sweet taste receptor stimulated by 12 sweeteners were investigated using functional cellular assays. Second, a total of 162 blood samples were collected from an obese population (BMI between 25 and 35 kg/m) involved in the SWEET project. The TaqMan method for SNP genotyping was carried out using DNA extracted from blood samples to identify new SNPs and predict possible/probable TAS1R2/TAS1R3 loss of function.
Although certain human / SNPs showed reduced receptor response, they were not associated with particular phenotypes. Seven SNPs were predicted to severely impair the human TAS1R2/TAS1R3 response to sweeteners.
Although some - and -SNPs have previously been associated with obesity, our cellular results do not confirm this association and reinforce the hypothesis, put forward by other researchers, that sweet taste perception and sugar consumption are governed by factors other than the and genes.
背景/目的：研究推测，TAS1R2和TAS1R3基因中的单核苷酸多态性（SNP）可能会改变甜味化合物的检测和饮食习惯，从而增加肥胖风险。这项体外研究旨在测定人类TAS1R2/TAS1R3多态性（其中一些被认为与肥胖有关）对甜味受体对各种甜味剂反应的影响。该研究还旨在在肥胖人群中鉴定与TAS1R2/TAS1R3功能降低或丧失相关的新SNP。
首先，使用功能性细胞试验研究了文献中先前鉴定的12种人类TAS1R2 - SNP和16种人类TAS1R3 - SNP对12种甜味剂刺激的甜味受体反应的影响。其次，从参与SWEET项目的肥胖人群（BMI在25至35kg/m之间）中总共采集了162份血样。使用从血样中提取的DNA进行SNP基因分型的TaqMan方法，以鉴定新的SNP并预测可能的TAS1R2/TAS1R3功能丧失。
尽管某些人类TAS1R2/TAS1R3 SNP显示受体反应降低，但它们与特定表型无关。预测有7个SNP会严重损害人类TAS1R2/TAS1R3对甜味剂的反应。
尽管先前一些TAS1R2和TAS1R3 - SNP与肥胖有关，但我们的细胞研究结果并未证实这种关联，而是强化了其他研究人员提出的假设，即甜味感知和糖消耗受TAS1R2和TAS1R3基因以外的因素控制。
