Nemazee D A, Studer S, Steinmetz M, Dembić Z, Kiefer M
Eur J Immunol. 1985 Aug;15(8):760-4. doi: 10.1002/eji.1830150804.
Mice bearing the recessive gene lpr develop an age-dependent, massive lymphoproliferation, primarily in the lymph nodes (LN), with associated autoimmunity. LN cells from these mice express T cell receptor protein on the cell surface at 50-70% of normal levels. Normal levels of T cell receptor alpha, beta and gamma mRNA were found in these cells as compared to normal LN cells. Southern blot analysis of MRL-lpr/lpr LN DNA showed rearrangements in 80-90% of the chromosomes at the beta gene loci. The pattern of rearrangement indicated that a polyclonal rather than monoclonal expansion of T cells occurred. These data support a lymphokine-induction model of lymphoproliferation in MRL-lpr mice.
携带隐性基因lpr的小鼠会出现与年龄相关的大量淋巴细胞增殖，主要发生在淋巴结（LN），并伴有自身免疫。这些小鼠的LN细胞在细胞表面表达的T细胞受体蛋白水平为正常水平的50%-70%。与正常LN细胞相比，在这些细胞中发现了正常水平的T细胞受体α、β和γ mRNA。对MRL-lpr/lpr LN DNA的Southern印迹分析显示，在β基因位点80%-90%的染色体中发生了重排。重排模式表明T细胞发生了多克隆而非单克隆扩增。这些数据支持MRL-lpr小鼠淋巴细胞增殖的淋巴因子诱导模型。
