Adams A T, Auersperg N
Exp Cell Biol. 1985;53(4):181-8. doi: 10.1159/000163311.
A cell line, ROSE 199, derived from rat ovarian surface epithelium (ROSE) formed papillary structures which resembled, histologically, serous papillary cystadenomas of borderline malignancy seen in the human ovary. Crowded cultures produced two layers of cells separated by a thick layer of collagen fibers. Such cultures shed viable cells into the growth medium, while no cells were shed by short-term ROSE cultures. The resemblance to ovarian tumors exhibited by ROSE 199 cells in culture, reinforces the hypothesis that the common epithelial tumors of the ovary are derived from the ovarian surface epithelium. ROSE 199 cells, while retaining their epithelial morphology and ultrastructural characteristics, express stromal activity such as abundant collagen production. Perhaps this ability to express epithelial and stromal behavior is a contributing factor to the ready neoplastic transformation of the ovarian surface epithelium.
一种源自大鼠卵巢表面上皮（ROSE）的细胞系ROSE 199形成了乳头状结构，从组织学上看，类似于人类卵巢中所见的交界性恶性浆液性乳头状囊腺瘤。密集培养产生了两层细胞，中间由一层厚厚的胶原纤维隔开。这种培养物会将活细胞释放到生长培养基中，而短期ROSE培养物则不会释放细胞。ROSE 199细胞在培养中与卵巢肿瘤的相似性，强化了卵巢常见上皮性肿瘤源自卵巢表面上皮的假说。ROSE 199细胞在保留其上皮形态和超微结构特征的同时，表现出基质活性，如大量产生胶原蛋白。也许这种表达上皮和基质行为的能力是卵巢表面上皮易于发生肿瘤转化的一个促成因素。
