de Farias Diana Maria, Leite Barros Flávia de Nazaré, Sampaio-Júnior Francisco Dantas, Dos Santos Cruz Vieira Juliana, de Sousa Gonçalves Thamirys, da Costa Rodrigues Amanda de Nazaré, de Lima Macedo Renata Cecília, Duarte Cerqueira Valíria, Mendes de Oliveira Ana Cristina, Souza da Paz Giselle, Góes-Cavalcante Gustavo, Scofield Alessandra
Laboratory of Animal Health, Institute of Veterinary Medicine, Federal University of Pará (UFPA), Castanhal, Brazil.
Vertebrate Zoology Laboratory, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Brazil.
Transbound Emerg Dis. 2023 Sep 26;2023:5943212. doi: 10.1155/2023/5943212. eCollection 2023.
is the etiological agent of histoplasmosis, which can infect birds and different mammal species, including humans. In Brazil, the disease is not notifiable, and little is known about its infection in domestic and wild mammals. This study aimed to perform the molecular detection of in small wild mammals from peri-urban forest remnants and in dogs and cats peri-domiciled in rural communities adjacent to these fragments in the Pará State, Brazilian Amazon. Samples of lung, liver, and skin were collected from free-living rats and marsupials captured in three peri-urban forest patches, as well as blood and skin from dogs and cats. DNA was detected by nested PCR amplification, with products sequenced and subjected to phylogenetic analysis. DNA was detected in 9.5% (12/126) of small wild mammals, with rats having a higher frequency of positive animals (25.6%; 10/39) when compared to marsupials (2.3%; 2/87) (=0.0001). The frequencies of positive dogs and cats were 1.6% (2/121) and 5.5% (1/18), respectively. A higher frequency of infection by was observed among small wild mammals when compared to dogs and cats (=0.0143). In conclusion, infection occurs in rats, marsupials, dogs, and cats in the Brazilian Amazon, with rats being important sentinels of the presence of this fungus in areas of remaining forest.
是组织胞浆菌病的病原体，可感染鸟类和包括人类在内的不同哺乳动物物种。在巴西，该病无需上报，人们对其在家养和野生哺乳动物中的感染情况了解甚少。本研究旨在对巴西亚马逊州帕拉州城市周边森林残余地的小型野生哺乳动物以及毗邻这些区域的农村社区周边家养的狗和猫进行分子检测。从在三个城市周边森林斑块捕获的自由生活的大鼠和有袋动物身上采集肺、肝和皮肤样本，以及从狗和猫身上采集血液和皮肤样本。通过巢式PCR扩增检测DNA，对产物进行测序并进行系统发育分析。在9.5%（12/126）的小型野生哺乳动物中检测到DNA，与有袋动物（2.3%；2/87）相比，大鼠的阳性动物频率更高（25.6%；10/39）（P=0.0001）。狗和猫的阳性频率分别为1.6%（2/121）和5.5%（1/18）。与狗和猫相比，在小型野生哺乳动物中观察到更高的感染频率（P=0.0143）。总之，在巴西亚马逊地区的大鼠、有袋动物、狗和猫中发生了感染，大鼠是剩余森林地区这种真菌存在的重要哨兵。
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