Iida Y
J Biochem. 1985 Apr;97(4):1173-9. doi: 10.1093/oxfordjournals.jbchem.a135162.
Many eukaryotic genes are interrupted by introns, which are removed from mRNA precursors by the RNA splicing mechanism. Although nucleotide sequences around splice sites have considerable homology among various genes, exact splice-site signals are unknown. Previously, applying the computer searching method to primary nucleotide sequences of various genes, we studied what kinds of patterns are the necessary and sufficient sequences for recognition of the 5'-slice site (donor site). We proposed that four common patterns, AG/GTA, /GTAAGT, RG/GTGAG, and AG/GTXXGT, where R = A or G and X = A, T, G, or C, are often used as such signals. In the present paper, we examined a number of experimental results on site-specific mutagenesis around donor sites and on alpha- and beta-thalassemia in globin genes, and found that the above four common patterns could explain almost all of those results.
许多真核基因被内含子打断，这些内含子通过RNA剪接机制从mRNA前体中去除。尽管不同基因的剪接位点周围的核苷酸序列具有相当高的同源性，但确切的剪接位点信号尚不清楚。以前，我们将计算机搜索方法应用于各种基因的初级核苷酸序列，研究了哪些模式是识别5'剪接位点（供体位点）的必要和充分序列。我们提出，四种常见模式AG/GTA、/GTAAGT、RG/GTGAG和AG/GTXXGT（其中R = A或G，X = A、T、G或C）常被用作此类信号。在本文中，我们研究了许多关于供体位点周围位点特异性诱变以及珠蛋白基因中α和β地中海贫血的实验结果，发现上述四种常见模式几乎可以解释所有这些结果。
