Zhang Ping, Han Ying, Xu Yue, Gao Liang
Department of Chemistry, College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China.
Biophys Rep. 2025 Apr 30;11(2):112-128. doi: 10.52601/bpr.2024.240039.
Biomacromolecules including proteins and nucleic acids are widely recognized for their pivotal and irreplaceable role in maintaining the normal functions of biological systems. By combining metal stable isotope labeling with elemental mass spectrometry, researchers can quantify the amount and track the spatial distribution of specific biomacromolecules in complex biological systems. In this review, the probes classification and metal stable isotope labeling strategies are initially summarized. Secondly, the technical characteristics and working principle of the elemental mass spectrometry techniques including inductively coupled plasma mass spectrometry and secondary ion mass spectrometry are introduced to achieve highly sensitive detection of multiple biomacromolecules at molecular, cellular and tissue levels. Lastly, we underline the advantages and limitations of elemental mass spectrometry combined with metal stable isotope labeling strategies, and propose the perspectives for future developments.
包括蛋白质和核酸在内的生物大分子因其在维持生物系统正常功能中所起的关键和不可替代的作用而被广泛认可。通过将金属稳定同位素标记与元素质谱相结合，研究人员可以在复杂生物系统中定量特定生物大分子的含量并追踪其空间分布。在本综述中，首先总结了探针分类和金属稳定同位素标记策略。其次，介绍了电感耦合等离子体质谱和二次离子质谱等元素质谱技术的技术特点和工作原理，以实现对分子、细胞和组织水平上多种生物大分子的高灵敏度检测。最后，我们强调了元素质谱结合金属稳定同位素标记策略的优点和局限性，并提出了未来发展的展望。
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