Payamani Masoumeh, Abdollahi-Keyvani Seyedeh-Tabassom, Sajjadi Mandana, Akhondzadeh Shahin, Javid Reihane, Nakhaei-Zadeh Reihaneh, Jalalian-Javadpour Marzieh, Vaseghi Salar
Cognitive Neuroscience Lab, Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.
Department of Global Studies, Faculty of World Studies, University of Tehran, Tehran, Iran.
Pharmacol Biochem Behav. 2025 Jul;252:174027. doi: 10.1016/j.pbb.2025.174027. Epub 2025 Apr 30.
Specific protocols of rapid-eye movement (REM) sleep deprivation (SD) may lead to behavioral phenotypes considered as obsessive-compulsive disorder (OCD)-like behavior and hyperactivity (mania-like behavior). The present study aimed to assess the effects of REM SD on both sexes of control and fear conditioning (FC) rats. REM SD was induced for 1 or 2 or 3 weeks (6 h/d). Freezing, locomotor activity, anxiety-like behavior, grooming and burying marbles (OCD-like behaviors), and brain-derived neurotrophic factor (BDNF) in the hippocampus were evaluated. The results showed 1w REM SD decreased freezing in females, while 2w and 3w REM SD decreased freezing in both sexes. Locomotor activity in 2w REM SD males was increased, while was decreased in 3w group. In females, both 2w and 3w REM SD increased locomotion. REM SD in both sexes increased locomotion in FC rats. All REM SD protocols decreased anxiety in both sexes of FC rats. REM SD in control and FC rats led to OCD-like behaviors. All REM SD protocols decreased BDNF in both sexes, while 3w slightly increased it, suggesting a compensatory mechanism over time. 2w and 3w REM SD increased BDNF in FC rats in both sexes. Pearson correlation test also showed that changes in BDNF levels may be related to some behavioral changes only in females. In conclusion, for the first time, the present study showed sex differences in the effects of REM SD on behavioral functions in control and FC rats, and in the relationship between BDNF levels and behavioral changes.
快速眼动（REM）睡眠剥夺（SD）的特定方案可能会导致被视为强迫症（OCD）样行为和多动（躁狂样行为）的行为表型。本研究旨在评估REM睡眠剥夺对对照和恐惧条件反射（FC）大鼠两性的影响。诱导REM睡眠剥夺1或2或3周（每天6小时）。评估了冻结行为、运动活动、焦虑样行为、梳理毛发和掩埋弹珠（OCD样行为）以及海马体中的脑源性神经营养因子（BDNF）。结果显示，1周的REM睡眠剥夺降低了雌性大鼠的冻结行为，而2周和3周的REM睡眠剥夺降低了两性大鼠的冻结行为。2周的REM睡眠剥夺使雄性大鼠的运动活动增加，而3周组则减少。在雌性大鼠中，2周和3周的REM睡眠剥夺均增加了运动活动。两性的REM睡眠剥夺均增加了FC大鼠的运动活动。所有REM睡眠剥夺方案均降低了FC大鼠两性的焦虑水平。对照和FC大鼠的REM睡眠剥夺导致了OCD样行为。所有REM睡眠剥夺方案均降低了两性的BDNF水平，而3周时略有增加，表明随着时间的推移存在一种补偿机制。2周和3周的REM睡眠剥夺增加了FC大鼠两性的BDNF水平。Pearson相关性检验还表明，BDNF水平的变化可能仅与雌性大鼠的某些行为变化有关。总之，本研究首次表明，REM睡眠剥夺对对照和FC大鼠行为功能的影响以及BDNF水平与行为变化之间的关系存在性别差异。
