Kar Ezgi, Övenler Zeynep, Hacıoğlu Ceyhan, Kar Fatih
Department of Nutrition and Dietetics, Faculty of Health Sciences, Kutahya Health Sciences University, Kutahya, Turkey.
Faculty of Medicine, Kutahya Health Sciences University, Kutahya, Turkey.
J Cell Mol Med. 2025 May;29(9):e70578. doi: 10.1111/jcmm.70578.
Glioblastoma is one of the deadliest cancers with a very low chance of survival. Glioblastomas have a poor prognosis because of their infiltrative nature, which makes them difficult to totally isolate with rigorous surgery, radiation, and chemotherapy. Our aim in this study was to investigate the efficacy of boric acid, which has anti-cancer properties, on glioblastoma, which has very limited treatment options. U251 human glioblastoma cell lines were treated with IC25 (15.62 μg/mL), IC50 (31.25 μg/mL) and IC75 (62.5 μg/mL) doses of boric acid. Cell viability and proliferation levels were tested. At the same time, the activity of boric acid on cells was tested through oxidative stress, apoptosis, and semaphorin signalling pathway parameters. Our findings indicate that boric acid induced dose-dependent oxidative stress, cellular growth inhibition, apoptosis and morphological changes in U251 cells. Additionally, treatments with increasing amounts of boric acid resulted in a rise in the production of biomarkers of the semaphorin pathway, which may limit cell growth and proliferation. We found that boric acid activates apoptosis by triggering ROS formation at high doses and at the same time inhibits cell proliferation by increasing semaphorin signalling pathway expressions. Boric acid may act as an anti-cancer agent by activating different mechanisms in a dose-dependent manner.
胶质母细胞瘤是最致命的癌症之一，生存率极低。由于其浸润性，胶质母细胞瘤预后较差，这使得通过严格的手术、放疗和化疗将其完全分离变得困难。我们在本研究中的目的是调查具有抗癌特性的硼酸对治疗选择非常有限的胶质母细胞瘤的疗效。用IC25（15.62μg/mL）、IC50（31.25μg/mL）和IC75（62.5μg/mL）剂量的硼酸处理U251人胶质母细胞瘤细胞系。测试细胞活力和增殖水平。同时，通过氧化应激、细胞凋亡和信号素信号通路参数测试硼酸对细胞的活性。我们的研究结果表明，硼酸在U251细胞中诱导了剂量依赖性的氧化应激、细胞生长抑制、细胞凋亡和形态变化。此外，用越来越多的硼酸处理导致信号素通路生物标志物的产生增加，这可能会限制细胞生长和增殖。我们发现，硼酸通过在高剂量时触发活性氧的形成来激活细胞凋亡，同时通过增加信号素信号通路的表达来抑制细胞增殖。硼酸可能通过以剂量依赖的方式激活不同机制而作为一种抗癌剂发挥作用。
