Binkley S, Mosher K, White B H
J Neurochem. 1985 Sep;45(3):875-8. doi: 10.1111/j.1471-4159.1985.tb04075.x.
N-Acetyltransferase (NAT) is an enzyme whose rhythmic activity in the pineal gland and retina is thought to be responsible for melatonin circadian rhythms. The enzyme has circadian properties--its rhythm persists in constant conditions, and it is precisely controlled by light and dark. Experiments are reported in which 4-h light or dark pulses were imposed on chicks (Gallus domesticus) over a 24-h period. Pineal NAT profiles were measured during and subsequent to the pulses. The phase of the NAT cycle following pulses was plotted to obtain phase-response curves. Light pulses produced a maximum phase shift (advance of 5 h) 8 h after the expected time of lights-out; dark pulses produced a maximum phase shift (advance of 4 h) 3 h after the expected time of lights-out. Maximum phase delays (-2 h) occurred 1-2 h after the expected lights-out for light pulses and 8 h after expected lights-on for dark pulses.
N-乙酰基转移酶（NAT）是一种酶，其在松果体和视网膜中的节律性活动被认为是褪黑素昼夜节律的原因。该酶具有昼夜节律特性——其节律在恒定条件下持续存在，并且受到光照和黑暗的精确控制。本文报道了在24小时内对小鸡（家鸡）施加4小时光照或黑暗脉冲的实验。在脉冲期间及之后测量松果体NAT谱。绘制脉冲后NAT周期的相位以获得相位响应曲线。光脉冲在预期熄灯时间后8小时产生最大相位偏移（提前5小时）；暗脉冲在预期熄灯时间后3小时产生最大相位偏移（提前4小时）。光脉冲的最大相位延迟（-2小时）发生在预期熄灯时间后1-2小时，暗脉冲的最大相位延迟发生在预期开灯时间后8小时。
