肌肉去神经支配会增加编码乙酰胆碱受体α亚基的两种mRNA的水平。

Muscle denervation increases the levels of two mRNAs coding for the acetylcholine receptor alpha-subunit.

作者信息

Goldman D, Boulter J, Heinemann S, Patrick J

出版信息

J Neurosci. 1985 Sep;5(9):2553-8. doi: 10.1523/JNEUROSCI.05-09-02553.1985.

DOI:10.1523/JNEUROSCI.05-09-02553.1985

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6565325/

Abstract

The mRNA coding for the alpha-subunit of the acetylcholine receptor was studied in mouse leg and rat diaphragm muscle. We find that denervation of rat diaphragm results in a 7-fold increase in mRNA coding for the alpha-subunit, whereas denervation of mouse leg muscle results in approximately a 50-fold increase in alpha-subunit-specific mRNA. The relationship of the mRNAs purified from innervated and denervated muscle was investigated by SI nuclease mapping. Two mRNA species were found in both innervated and denervated muscle which differ in their 3'-untranslated region. The levels of both these mRNA species increase upon denervation of mouse leg muscle.

摘要

对小鼠腿部肌肉和大鼠膈肌中编码乙酰胆碱受体α亚基的信使核糖核酸（mRNA）进行了研究。我们发现，大鼠膈肌去神经支配后，编码α亚基的mRNA增加了7倍，而小鼠腿部肌肉去神经支配后，α亚基特异性mRNA增加了约50倍。通过核酸酶S1图谱分析研究了从神经支配和去神经支配肌肉中纯化的mRNA之间的关系。在神经支配和去神经支配的肌肉中均发现了两种mRNA，它们的3'非翻译区不同。小鼠腿部肌肉去神经支配后，这两种mRNA的水平均升高。

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