Gu Meiqi, Li Ting, Pan Pinghui, Massawe Aneth, Fang Chuandong, Tu Chuanhai, Liu Zhiyu, Zhang Bin
College of Food Science and Pharmacy, Zhejiang Ocean University, Zhoushan 316022, PR China.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
Food Chem X. 2025 Apr 16;27:102465. doi: 10.1016/j.fochx.2025.102465. eCollection 2025 Apr.
This study investigated the changes in physicochemical properties and microbial diversity during the fermentation of low-salt shrimp paste with the addition of different carbon sources (glucose and glutinous rice flour). Notably, during fermentation, the low-salt shrimp paste with glucose and glutinous rice flour had lower TVB-N values and higher amino acid nitrogen (AAN) values, which may be responsible for increasing the flavor of the low-salt shrimp paste compared to the control group. Addition of glucose and glutinous rice flour to low-salt shrimp paste is beneficial in inhibiting histamine production. High-throughput sequencing showed that at the family level, the relative abundance of Bacillaceae at the end of fermentation was 63.89 % in the control group, 57.31 % in the group with the addition of glucose, and 44.18 % in the group with the addition of glutinous rice flour. The addition of glucose and glutinous rice flour reduced the relative abundance of Bacillaceae, which were partially pathogenic. Changes in the physicochemical properties were associated with various microorganisms, which collectively impacted the quality of low-salt shrimp paste. This study provides a theoretical reference for improving the quality of shrimp paste, reducing the cost of shrimp paste, and further advancing the fermentation process of novel low-salt shrimp paste.
本研究考察了添加不同碳源(葡萄糖和糯米粉)的低盐虾酱发酵过程中理化性质和微生物多样性的变化。值得注意的是,在发酵过程中,添加葡萄糖和糯米粉的低盐虾酱TVB-N值较低,氨基酸态氮(AAN)值较高,这可能是其相比对照组低盐虾酱风味增加的原因。向低盐虾酱中添加葡萄糖和糯米粉有利于抑制组胺的产生。高通量测序表明,在科水平上,发酵结束时对照组芽孢杆菌科的相对丰度为63.89%,添加葡萄糖组为57.31%,添加糯米粉组为44.18%。葡萄糖和糯米粉的添加降低了部分具有致病性的芽孢杆菌科的相对丰度。理化性质的变化与多种微生物有关,这些微生物共同影响了低盐虾酱的品质。本研究为提高虾酱品质、降低虾酱成本以及进一步推进新型低盐虾酱的发酵工艺提供了理论参考。