Csorba Artúr Botond, Szanyi Kálmán, Szanyi Szabolcs, Tarcali Gábor, Balog Adalbert, Nagy Antal
Department of Horticulture, Faculty of Technical and Human Sciences, Sapientia Hungarian University of Transylvania, Aleea Sighișoarei 2, Târgu Mureș, 540485 Corunca, Romania.
Faculty of the Agricultural and Food Sciences and Environmental Management, Institute of Plant Protection, University of Debrecen, Böszörményi Str. 138, 4032 Debrecen, Hungary.
Insects. 2025 Apr 15;16(4):417. doi: 10.3390/insects16040417.
During this research, the corn leaf aphids endosymbiotic bacterial diversity was tested in the same crop systems (monoculture industrial maize as grain for livestock) and the same soil type (Chernozem) when pre-crop pesticide management was used. Bacterial symbionts were analyzed using Illumina systems, and the Silva 16S NR99 V138.2 database was used to assign bacterial taxa on genus and species levels. The presence of the obligate endosymbiont has been clearly detected in all cases, and in all samples but its abundance varied between samples inside crops, but not between crops and generations. The facultative symbionts and spp. frequency varied between generations, and increased at generation II; however, differences were not significant. We concluded that the pre-crop pesticide application has no effect on corn leaf aphids bacterial symbionts, so the indirect pesticide application on aphids adaptation is low or nonexistent.
在这项研究中,当采用作物前农药管理时,在相同的作物系统(作为牲畜饲料谷物的单一栽培工业玉米)和相同的土壤类型(黑钙土)中测试了玉米叶蚜内共生细菌的多样性。使用Illumina系统分析细菌共生体,并使用Silva 16S NR99 V138.2数据库在属和种水平上确定细菌分类群。在所有情况下和所有样本中都已明确检测到专性内共生体的存在,但其丰度在作物内部的样本之间有所不同,但在作物和世代之间没有差异。兼性共生体和 spp. 的频率在世代之间有所变化,并在第二代增加;然而,差异不显著。我们得出结论,作物前施用农药对玉米叶蚜细菌共生体没有影响,因此农药对蚜虫适应性的间接影响很低或不存在。