Hazel J R
Lipids. 1985 Aug;20(8):516-20. doi: 10.1007/BF02534892.
The phospholipid composition of gill tissue from thermally acclimated rainbow trout, Salmo gairdneri, was determined by Iatroscan analysis following an initial development of the chromarods in a non-polar solvent to remove neutral lipids. Standard curves for all phospholipids, although linear through most of the concentration range tested (1-40 micrograms), extrapolated to negative intercepts on the ordinate, indicating a decline in sensitivity at low phospholipid levels. In addition, the concentration dependence of the Iatroscan response varied by nearly 6-fold among phospholipids. Of the major phospholipids, only lysophosphatidylcholine could not be quantitated accurately because of the presence of an interfering peak. Quantitation by Iatroscan yielded results which, in general, agreed well (within 5%) with results obtained by an independent phosphate analysis. Only in the case of phosphatidylinositol (PI) did the two analytical methods differ significantly; proportions of PI were 55% higher when determined by Iatroscan as opposed to phosphate analysis. Gill tissue from 5 C-acclimated trout possessed higher proportions of phosphatidylethanolamine than tissue from 20 C-acclimated trout. The Iatroscan provided a rapid and reliable means of quantitating the proportions of all the major phospholipids of trout gill, although there are some limitations to the general applicability of the technique.
在非极性溶剂中初步展开色棒以去除中性脂质后，通过Iatroscan分析测定了热适应虹鳟（Salmo gairdneri）鳃组织的磷脂组成。所有磷脂的标准曲线虽然在测试的大部分浓度范围（1 - 40微克）内呈线性，但外推至纵坐标上的负截距，表明在低磷脂水平下灵敏度下降。此外，Iatroscan响应的浓度依赖性在不同磷脂之间相差近6倍。在主要磷脂中，由于存在干扰峰，只有溶血磷脂酰胆碱无法准确定量。通过Iatroscan进行定量得到的结果总体上与通过独立的磷酸盐分析获得的结果吻合良好（在5%以内）。只有在磷脂酰肌醇（PI）的情况下，这两种分析方法存在显著差异；通过Iatroscan测定时PI的比例比通过磷酸盐分析测定时高55%。5℃适应的鳟鱼鳃组织中磷脂酰乙醇胺的比例高于20℃适应的鳟鱼组织。Iatroscan提供了一种快速且可靠的方法来定量鳟鱼鳃中所有主要磷脂的比例，尽管该技术的普遍适用性存在一些局限性。
