Xie Long, Huang Liqun, Fang Xueting, Zha Jinshun, Su Yingrui
Department of Nuclear Medicine, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
Department of Pathology, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian, China.
PLoS One. 2025 May 7;20(5):e0323531. doi: 10.1371/journal.pone.0323531. eCollection 2025.
To evaluate liver function using dynamic hepatobiliary single-photon emission computed tomography (SPECT) in different rat models of acute liver failure.
Twenty-four 6-8-week-old male Sprague-Dawley rats (weight 190-200 g) were evenly divided into four groups. Acute liver failure was induced by intraperitoneal injection of D-galactosamine (D-GalN, 600 mg/kg) and lipopolysaccharide (LPS, 10 µg/kg), common bile duct ligation surgery, and removing 70% of the liver mass. The fourth group served as the control without intervention. The time-activity curves for the liver and heart were generated from dynamic SPECT scans with 99mTc-ethylene hepatobiliary iminodiacetic acid (EHIDA). Image-derived functional parameters (5-minute heart/liver index [HLI5] and 15-minute receptor index [LHL15]) were calculated. Furthermore, correlations of image-derived parameters with serum interleukin-6 (IL-6) levels, liver aspartate aminotransferase (AST) and alanine transaminase (ALT) levels, and liver mRNA expression levels of tumor necrosis factor-α (TNF-α) and chemokine ligand-10 (CXCL-10) were analyzed.
All animals in the experimental groups exhibited varying degrees of liver damage. The SPECT images and indexes (HLI5 and LHL15) of the experimental groups significantly differed from those of the control group (P < 0.05). In the experimental groups, serum IL-6 levels and liver mRNA levels of TNF-α and CXCL-10 were significantly higher, while liver AST and ALT levels were significantly lower than those in the control group (P < 0.05).
Using SPECT with 99mTc-EHIDA, along with the calculated indexes and levels of various cytokines, presents a dependable method for assessing liver function.
在不同的急性肝衰竭大鼠模型中，使用动态肝胆单光子发射计算机断层扫描（SPECT）评估肝功能。
将24只6 - 8周龄雄性Sprague-Dawley大鼠（体重190 - 200 g）平均分为四组。通过腹腔注射D-半乳糖胺（D-GalN，600 mg/kg）和脂多糖（LPS，10 μg/kg）、胆总管结扎手术以及切除70%的肝脏质量来诱导急性肝衰竭。第四组作为未干预的对照组。使用99mTc-乙基亚氨基二乙酸（EHIDA）通过动态SPECT扫描生成肝脏和心脏的时间-活性曲线。计算图像衍生的功能参数（5分钟心脏/肝脏指数 [HLI5] 和15分钟受体指数 [LHL15]）。此外，分析图像衍生参数与血清白细胞介素-6（IL-6）水平、肝脏天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平以及肿瘤坏死因子-α（TNF-α）和趋化因子配体-10（CXCL-10）的肝脏mRNA表达水平之间的相关性。
实验组所有动物均表现出不同程度的肝损伤。实验组的SPECT图像和指标（HLI5和LHL15）与对照组有显著差异（P < 0.05）。在实验组中，血清IL-6水平以及TNF-α和CXCL-10的肝脏mRNA水平显著高于对照组，而肝脏AST和ALT水平显著低于对照组（P < 0.05）。
使用99mTc-EHIDA的SPECT以及计算得出的指标和各种细胞因子水平，为评估肝功能提供了一种可靠的方法。
