Ramos Gabriela Rocha, Ostrosky Elissa Arantes, Lopes Patrícia Santos, Filho Newton Andréo, Kakuda Lohanna Luciyanla, da Silva Pinto Jéssica Nascimento, Viana Emanuela de Lima, Morais Neyna Santos, Rios Nathalia Saraiva, Barreto Gomes Ana Paula, Júnior Francisco Humberto Xavier, Júnior Francisco Canindé de Sousa, de Assis Cristiane Fernandes
Pharmaceutical Sciences Postgraduate Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Department of Pharmacy, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, RN, Brazil.
PLoS One. 2025 May 7;20(5):e0322106. doi: 10.1371/journal.pone.0322106. eCollection 2025.
This study aimed to evaluate the potential of lipase from Aspergillus terreus as an active ingredient in cosmetic formulations. Lipase was produced using the fungus Aspergillus terreus and was immobilized on gel silica as support. The enzymes were characterized using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Thermogravimetry and Differential Scanning Calorimetry (TG/DSC), and safety evaluation through cytotoxicity tests using NIH-3T3 fibroblast cells. A central composite rotatable design was employed to find the best conditions for enzymatic cosmetic production. The enzyme produced by A. terreus showed activity of 375.9 U/g of substrate, and the immobilized enzyme showed 12.78 U/g of silica, while the lipase from R. oryzae showed activity of 69.91 U/g. As confirmed by FTIR and XRD, SEM showed weak enzyme interaction with silica during immobilization. Cytotoxicity tests showed that only the lipase produced by A. terreus was safe for NIH-3T3 fibroblast cells. The central composite rotatable design showed the agitation time influenced the enzyme activity response. According to the results, the enzyme produced by the fungus A. terreus is a promising and safe product for research into developing new cosmetic products.
本研究旨在评估土曲霉脂肪酶作为化妆品配方中活性成分的潜力。脂肪酶是利用土曲霉生产的，并固定在硅胶上作为载体。使用扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、热重分析和差示扫描量热法（TG/DSC）对酶进行表征，并通过使用NIH-3T3成纤维细胞的细胞毒性试验进行安全性评估。采用中心复合旋转设计来寻找酶促化妆品生产的最佳条件。土曲霉产生的酶显示出375.9 U/g底物的活性，固定化酶显示出12.78 U/g硅胶的活性，而米根霉脂肪酶显示出69.91 U/g的活性。如FTIR和XRD所证实，SEM显示固定化过程中酶与硅胶的相互作用较弱。细胞毒性试验表明，只有土曲霉产生的脂肪酶对NIH-3T3成纤维细胞是安全的。中心复合旋转设计表明搅拌时间影响酶活性响应。根据结果，土曲霉产生的酶是开发新型化妆品研究中一种有前景且安全的产品。
